Department of Electrical and Computer Engineering, Micro and Nanotechnology Laboratory, University of Illinois, Urbana, IL 61801, USA.
Lab Chip. 2011 Apr 21;11(8):1437-47. doi: 10.1039/c0lc00556h. Epub 2011 Feb 1.
We have developed a microfabricated biochip that enumerates CD4+ T lymphocytes from leukocyte populations obtained from human blood samples using electrical impedance sensing and immunoaffinity chromatography. T cell counts are found by obtaining the difference between the number of leukocytes before and after depleting CD4+ T cells with immobilized antibodies in a capture chamber. This differential counting technique has been validated to analyze physiological concentrations of leukocytes with an accuracy of ∼9 cells per µL by passivating the capture chamber with bovine serum albumin. In addition, the counter provided T cell counts which correlated closely with an optical control (R(2) = 0.997) for CD4 cell concentrations ranging from approximately 100 to 700 cells per µL. We believe that this approach can be a promising method for bringing quantitative HIV/AIDS diagnostics to resource-poor regions in the world.
我们开发了一种微加工生物芯片，使用电阻抗感应和免疫亲和层析技术从人类血液样本中的白细胞群体中计数 CD4+T 淋巴细胞。通过在捕获室中用固定化抗体耗尽 CD4+T 细胞，获得白细胞数量在耗尽前后的差异，从而获得 T 细胞计数。通过用牛血清白蛋白使捕获室钝化，该差分计数技术已被验证可分析生理浓度的白细胞，其精度约为每 µL 9 个细胞。此外，该计数器提供的 T 细胞计数与光学对照（R²=0.997）非常吻合，用于 CD4 细胞浓度约为每 µL 100 至 700 个细胞的范围。我们相信，这种方法可能是为世界资源匮乏地区带来定量 HIV/AIDS 诊断的有前途的方法。
