Ferro M E, Galmarini M, Riera C M
Am J Reprod Immunol Microbiol. 1986 Aug;11(4):112-7. doi: 10.1111/j.1600-0897.1986.tb00043.x.
The injection of the spleen mononuclear (SpM) cells, obtained from rats rendered unresponsive to autoantigen of rat male accessory glands (RAG) by pretreatment with low doses of purified fraction of RAG (containing the autoantigen), into normal syngeneic recipients markedly prevented the development of delayed type hypersensitivity (DTH) reaction to the autoantigen (suppression of the induction) (p less than 0.001). The humoral response was not altered. The control animals were rat receptors of spleen mononuclear cells from donor rats pretreated with rat lung saline extract or 0.15 M NaCl. In contrast, the transference of SpM cells from donor rats pretreated with low doses of autoantigen prior to the immunization to rats previously immunized, did not modify the expression of the immune response against the autoantigen when compared with control rats. The suppression of the induction of DTH response was also obtained when prior to the immunization, the recipients received T-cell-enriched SpM cell population from unresponsive animals (p less than 0.001), but not when they were injected with B-cell-enriched SpM cells. These results suggest that suppressor T cells capable of controlling induction of the autoimmune response against RAG autoantigen might be one of the immunoregulatory mechanisms that are activated when soluble autoantigen of RAG enter into circulation.
将经低剂量纯化的大鼠雄性副腺自身抗原（RAG）组分（含自身抗原）预处理后对RAG自身抗原无反应的大鼠的脾单核细胞（SpM）注入正常同基因受体，可显著预防对该自身抗原的迟发型超敏反应（DTH）的发生（诱导抑制）（p<0.001）。体液反应未改变。对照动物为接受经大鼠肺盐水提取物或0.15M NaCl预处理的供体大鼠的脾单核细胞的大鼠受体。相比之下，将免疫前经低剂量自身抗原预处理的供体大鼠的SpM细胞转移至先前已免疫的大鼠，与对照大鼠相比，并未改变针对自身抗原的免疫反应的表达。当受体在免疫前接受来自无反应动物的富含T细胞的SpM细胞群体时，也可获得DTH反应诱导的抑制（p<0.001），但当给它们注射富含B细胞的SpM细胞时则不然。这些结果表明，能够控制针对RAG自身抗原的自身免疫反应诱导的抑制性T细胞可能是当RAG可溶性自身抗原进入循环时被激活的免疫调节机制之一。
