Logemann J, Lipphardt S, Lörz H, Häuser I, Willmitzer L, Schell J
Max-Planck-Institut für Züchtungsforschung, Köln, Federal Republic of Germany.
Plant Cell. 1989 Jan;1(1):151-8. doi: 10.1105/tpc.1.1.151.
A 1.2-kilobase pair fragment of the 5' upstream region of a potato wound-inducible gene (wun1) was fused to different marker genes (wun1-CAT, wun1-NPTII). Stable integration of a wun1-CAT chimeric gene into the tobacco genome led to a high wound-inducible chloramphenicol acetyltransferase activity in leaves. Transient expression experiments in potato protoplasts showed that wun1 carries a strong promoter sequence similar in strength to the 35S promoter. The same intensity of expression was also observed using wun1 constructs in transient experiments with rice protoplasts. wun1 mRNA was shown to accumulate to high levels in potato leaves collapsing as a result of infection with the phytopathogen Phytophthora infestans. The wun1 product might, therefore, play a role in a general physiological reaction to stress correlated with cell death.
将马铃薯创伤诱导基因（wun1）5'上游区域的一个1.2千碱基对片段与不同的标记基因（wun1-CAT、wun1-NPTII）融合。wun1-CAT嵌合基因稳定整合到烟草基因组中，导致叶片中创伤诱导的氯霉素乙酰转移酶活性很高。在马铃薯原生质体中进行的瞬时表达实验表明，wun1带有一个强度与35S启动子相似的强启动子序列。在水稻原生质体的瞬时实验中使用wun1构建体时，也观察到了相同强度的表达。结果表明，wun1 mRNA在因感染植物病原菌致病疫霉而萎蔫的马铃薯叶片中积累到高水平。因此，wun1产物可能在与细胞死亡相关的应激一般生理反应中发挥作用。
