Constabel C P, Bertrand C, Brisson N
Department of Biochemistry, Université de Montréal, Canada.
Plant Mol Biol. 1993 Aug;22(5):775-82. doi: 10.1007/BF00027364.
The STH-2 gene is rapidly activated in potato leaves and tubers following elicitation or infection by Phytophthora infestans. However, its biochemical function remains unknown. In order to ascertain if STH-2 protein is directly involved in the defense of potato against pathogens, the STH-2 coding sequence under the control of the CaMV 35S promoter was introduced into potato plants. Transgenic plants expressing the STH-2 gene were analyzed for an altered pattern of susceptibility to a compatible race of P. infestans and to potato virus X. Results indicate that constitutive expression of the STH-2 gene did not reduce susceptibility of potato to these pathogens.
在马铃薯叶片和块茎受到致病疫霉诱导或感染后，STH - 2基因会迅速被激活。然而，其生化功能仍然未知。为了确定STH - 2蛋白是否直接参与马铃薯对病原体的防御，将受花椰菜花叶病毒35S启动子控制的STH - 2编码序列导入马铃薯植株。对表达STH - 2基因的转基因植株进行分析，以检测其对致病疫霉的一个亲和小种和马铃薯X病毒的易感性模式是否改变。结果表明，STH - 2基因的组成型表达并未降低马铃薯对这些病原体的易感性。
