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1
Constitutive expression of pathogenesis-related proteins PR-1, GRP, and PR-S in tobacco has no effect on virus infection.病程相关蛋白PR-1、GRP和PR-S在烟草中的组成型表达对病毒感染没有影响。
Plant Cell. 1989 Mar;1(3):285-91. doi: 10.1105/tpc.1.3.285.
2
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3
Analysis of regulatory elements involved in the induction of two tobacco genes by salicylate treatment and virus infection.水杨酸处理和病毒感染诱导两个烟草基因过程中涉及的调控元件分析
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8
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Zucchini yellow mosaic virus-induced hypersensitive response is associated with pathogenesis-related 1 protein expression and confers resistance in watermelon.南瓜花叶病毒诱导的过敏反应与病程相关蛋白 1 的表达有关，并赋予西瓜抗性。
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本文引用的文献

1
A simple and general method for transferring genes into plants.一种将基因转入植物的简单而通用的方法。
Science. 1985 Mar 8;227(4691):1229-31. doi: 10.1126/science.227.4691.1229.
2
Antifungal Hydrolases in Pea Tissue : II. Inhibition of Fungal Growth by Combinations of Chitinase and beta-1,3-Glucanase.豌豆组织中的抗真菌水解酶：II.几丁质酶和β-1,3-葡聚糖酶组合对真菌生长的抑制作用。
Plant Physiol. 1988 Nov;88(3):936-42. doi: 10.1104/pp.88.3.936.
3
Characterization of osmotin : a thaumatin-like protein associated with osmotic adaptation in plant cells.渗透素的特性：一种与植物细胞渗透适应相关的类甜蛋白。
Plant Physiol. 1987 Oct;85(2):529-36. doi: 10.1104/pp.85.2.529.
4
Regulation of a plant pathogenesis-related enzyme: Inhibition of chitinase and chitinase mRNA accumulation in cultured tobacco tissues by auxin and cytokinin.植物病程相关酶的调节：生长素和细胞分裂素对培养烟草组织中几丁质酶和几丁质酶 mRNA 积累的抑制作用。
Proc Natl Acad Sci U S A. 1987 Jan;84(1):89-93. doi: 10.1073/pnas.84.1.89.
5
Biological function of pathogenesis-related proteins: Four tobacco pathogenesis-related proteins are chitinases.致病相关蛋白的生物学功能：四种烟草致病相关蛋白为几丁质酶。
Proc Natl Acad Sci U S A. 1987 Oct;84(19):6750-4. doi: 10.1073/pnas.84.19.6750.
6
Biological function of ;pathogenesis-related' proteins: four PR proteins of tobacco have 1,3-beta-glucanase activity."pathogenesis-related" 蛋白的生物学功能：烟草中的 4 种 PR 蛋白具有 1,3-β-葡聚糖酶活性。
EMBO J. 1987 Nov;6(11):3209-12. doi: 10.1002/j.1460-2075.1987.tb02637.x.
7
cDNA cloning of six mRNAs induced by TMV infection of tobacco and a characterization of their translation products.烟草花叶病毒（TMV）感染烟草诱导产生的六种mRNA的cDNA克隆及其翻译产物的特性分析
EMBO J. 1986 Sep;5(9):2057-61. doi: 10.1002/j.1460-2075.1986.tb04466.x.
8
Molecular characterization of messenger RNAs for 'pathogenesis related' proteins la, lb and lc, induced by TMV infection of tobacco.烟草花叶病毒感染烟草诱导产生的“病程相关”蛋白1a、1b和1c信使核糖核酸的分子特征
EMBO J. 1986 Jan;5(1):37-40. doi: 10.1002/j.1460-2075.1986.tb04174.x.
9
Virus-induced synthesis of messenger RNAs for precursors of pathogenesis-related proteins in tobacco.病毒诱导烟草中病程相关蛋白前体信使核糖核酸的合成。
EMBO J. 1985 Sep;4(9):2167-71. doi: 10.1002/j.1460-2075.1985.tb03911.x.
10
Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.用于革兰氏阴性菌的广宿主范围DNA克隆系统：苜蓿根瘤菌基因文库的构建
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7347-51. doi: 10.1073/pnas.77.12.7347.

病程相关蛋白PR-1、GRP和PR-S在烟草中的组成型表达对病毒感染没有影响。

Constitutive expression of pathogenesis-related proteins PR-1, GRP, and PR-S in tobacco has no effect on virus infection.

作者信息

Linthorst H J, Meuwissen R L, Kauffmann S, Bol J F

机构信息

Department of Biochemistry, State University of Leiden, The Netherlands.

出版信息

Plant Cell. 1989 Mar;1(3):285-91. doi: 10.1105/tpc.1.3.285.

DOI:10.1105/tpc.1.3.285
PMID:2535503
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC159761/
Abstract

Samsun NN tobacco cells were transformed with chimeric genes for pathogenesis-related (PR) proteins derived from genomic (PR-1a, GRP) or cDNA (PR-S) clones under the transcriptional control of the cauliflower mosaic virus 35S promoter. Regenerated plants were assayed by RNA and protein gel blotting, and plants showing high specific expression of the inserted genes were selected for self-pollination and seed formation. Inspection of second generation transformants showed that constitutive expression of PR-1a, GRP, and PR-S in tobacco in general does not have an effect on the phenotypic appearance of the plants or the expression of other endogenous PR genes. Furthermore, constitutive expression of the above genes does not affect the susceptibility of the plants to infection with tobacco mosaic virus or alfalfa mosaic virus.

摘要

用来自基因组（PR-1a、GRP）或cDNA（PR-S）克隆的病程相关（PR）蛋白的嵌合基因转化萨姆松NN烟草细胞，这些基因受花椰菜花叶病毒35S启动子的转录控制。通过RNA和蛋白质凝胶印迹分析再生植株，选择显示插入基因高特异性表达的植株进行自花授粉和种子形成。对第二代转化体的检查表明，PR-1a、GRP和PR-S在烟草中的组成型表达一般对植株的表型外观或其他内源性PR基因的表达没有影响。此外，上述基因的组成型表达不影响植株对烟草花叶病毒或苜蓿花叶病毒感染的敏感性。