Liu Li, Le Yang, Jin Juliang, Zhou Yuliang, Chen Guowei
Department of Civil Engineering, Hefei University of Technology, Tunxi Road 193, Hefei, 230009, China.
Appl Microbiol Biotechnol. 2015 Mar;99(6):2861-9. doi: 10.1007/s00253-014-6166-9. Epub 2014 Oct 31.
Microbial attachment to drinking water pipe surfaces facilitates pathogen survival and deteriorates disinfection performance, directly threatening the safety of drinking water. Notwithstanding that the formation of biofilm has been studied for decades, the underlying mechanisms for the origins of microbial surface attachment in biofilm development in drinking water pipelines remain largely elusive. We combined experimental and mathematical methods to investigate the role of environmental stress-mediated cell motility on microbial surface attachment in chlorination-stressed drinking water distribution systems. Results show that at low levels of disinfectant (0.0-1.0 mg/L), the presence of chlorine promotes initiation of microbial surface attachment, while higher amounts of disinfectant (>1.0 mg/L) inhibit microbial attachment. The proposed mathematical model further demonstrates that chlorination stress (0.0-5.0 mg/L)-mediated microbial cell motility regulates the frequency of cell-wall collision and thereby controls initial microbial surface attachment. The results reveal that transport processes and decay patterns of chlorine in drinking water pipelines regulate microbial cell motility and, thus, control initial surface cell attachment. It provides a mechanistic understanding of microbial attachment shaped by environmental disinfection stress and leads to new insights into microbial safety protocols in water distribution systems.
微生物附着在饮用水管道表面会促进病原体存活并降低消毒效果，直接威胁饮用水安全。尽管生物膜的形成已被研究了数十年，但饮用水管道生物膜发育过程中微生物表面附着起源的潜在机制仍 largely 难以捉摸。我们结合实验和数学方法，研究了环境应激介导的细胞运动在氯化应激饮用水分配系统中微生物表面附着中的作用。结果表明，在低浓度消毒剂（0.0 - 1.0 mg/L）下，氯的存在促进微生物表面附着的起始，而较高量的消毒剂（>1.0 mg/L）则抑制微生物附着。所提出的数学模型进一步表明，氯化应激（0.0 - 5.0 mg/L）介导的微生物细胞运动调节细胞壁碰撞频率，从而控制初始微生物表面附着。结果表明，饮用水管道中氯的传输过程和衰减模式调节微生物细胞运动，进而控制初始表面细胞附着。它提供了对由环境消毒应激塑造的微生物附着的机制理解，并为供水系统中的微生物安全协议带来了新的见解。
