Stem cell Engineering Research Center, School of Medicine, Anhui University of Science and Technology Huainan 232001, P.R. China ; The State Key Laboratory of Virology, Life Sciences College, Wuhan University Wuhan, Hubei 430072, P.R. China.
Department of Clinical Laboratory, Medical College, Hunan Normal University Changsha 410006, Hunan, China.
Am J Transl Res. 2014 Oct 11;6(5):528-37. eCollection 2014.
The efficient delivery of therapeutic molecule agents into target cells of interest is a critical challenge to broad application of non-viral vector systems. In this research, maytansine-loaded star-shaped folate-core polylactide-D-α-tocopheryl polyethylene glycol 1000 succinate (FA-PLA-TPGS) block copolymer was applied to be a vector of maytansine for folate receptor positive (FR(+)) breast cancer therapy. The uptake of maytansine nanoparticles by SKBR3 cells were observed by fluorescence microscopy and confocal laser scanning microscopy. The cell viability of maytansine-NPs in SKBR3 cells was assessed according to the changed level of intracellular microtubules and apoptosis-associated proteins. The cytotoxicity of the SKBR3 cells was significantly increased by maytansine-NPs when compared with control groups. In conclusion, the maytansine-NPs offer a considerable potential formulation for FR-expressing tumor targeting biotherapy.
载药星形叶酸核聚乳酸-聚乙二醇 1000 琥珀酸酯（FA-PLA-TPGS）嵌段共聚物作为载药系统递送至叶酸受体阳性（FR(+））乳腺癌细胞内，用于治疗多烯紫杉醇。通过荧光显微镜和共聚焦激光扫描显微镜观察载药纳米粒进入 SKBR3 细胞的情况。根据细胞内微管和凋亡相关蛋白的变化水平评估 SKBR3 细胞中多烯紫杉醇纳米粒的细胞活力。与对照组相比，多烯紫杉醇纳米粒明显增加了 SKBR3 细胞的细胞毒性。总之，多烯紫杉醇纳米粒为 FR 表达肿瘤靶向生物治疗提供了一种很有前途的制剂。
