Leroux Charles-Edouard, Monnier Sylvain, Wang Irène, Cappello Giovanni, Delon Antoine
Univ. Grenoble Alpes, LIPHY, F-38000 Grenoble, France ; CNRS, LIPHY, F-38000 Grenoble, France ; Institut Langevin, ESPCI ParisTech, CNRS, 1 rue Jussieu, 75238 Paris Cedex 05, France.
UMR 168, Institut Curie, Centre de Recherche, 26 rue d'Ulm 75005 Paris, France.
Biomed Opt Express. 2014 Sep 29;5(10):3730-8. doi: 10.1364/BOE.5.003730. eCollection 2014 Oct 1.
In this study we demonstrate the use of adaptive optics to correct the biasing effects of optical aberrations when measuring the dynamics of molecules diffusing between cells in multicellular spheroids. Our results indicate that, on average, adaptive optics leads to a reduction of the 3D size of the point spread function that is statistically significant in terms of measured number of molecules and diffusion time. The sensorless approach, which uses the molecular brightness as optimization metric, is validated in a complex, highly heterogeneous, biological environment. This work paves the way towards the design of accurate diffusion measurements of molecules in thick biological specimens.
在本研究中，我们展示了在测量多细胞球体中细胞间扩散分子的动力学时，使用自适应光学技术来校正光学像差的偏置效应。我们的结果表明，平均而言，自适应光学技术会使点扩散函数的三维尺寸减小，这在测量的分子数量和扩散时间方面具有统计学意义。以分子亮度作为优化指标的无传感器方法，在复杂、高度异质的生物环境中得到了验证。这项工作为在厚生物标本中精确测量分子扩散的设计铺平了道路。
