Hemmatzadeh Farhid, Boardman Wayne, Alinejad Arezo, Hematzade Azar, Moghadam Majid Kharazian
School of Animal and Veterinary Sciences, The University of Adelaide, Adelaide, Australia.
School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, Australia.
PLoS One. 2016 Dec 20;11(12):e0168756. doi: 10.1371/journal.pone.0168756. eCollection 2016.
A molecular and serological survey of selected viruses in free-ranging wild ruminants was conducted in 13 different districts in Iran. Samples were collected from 64 small wild ruminants belonging to four different species including 25 Mouflon (Ovis orientalis), 22 wild goat (Capra aegagrus), nine Indian gazelle (Gazella bennettii) and eight Goitered gazelle (Gazella subgutturosa) during the national survey for wildlife diseases in Iran. Serum samples were evaluated using serologic antibody tests for Peste de petits ruminants virus (PPRV), Pestiviruses [Border Disease virus (BVD) and Bovine Viral Diarrhoea virus (BVDV)], Bluetongue virus (BTV), Bovine herpesvirus type 1 (BHV-1), and Parainfluenza type 3 (PI3). Sera were also ELISA tested for Pestivirus antigen. Tissue samples including spleen, liver, lung, tonsils, mesenteric and mediastinal lymph nodes and white blood cells (WBCs) were tested using polymerase chain reaction (PCR) for PPRV, Foot and Mouth Disease virus (FMDV), Pestivirus, BTV, Ovine herpesvirus type 2 (OvHV-2) and BHV-1. Serologic tests were positive for antibodies against PPRV (17%), Pestiviruses (2%) and BTV (2%). No antibodies were detected for BHV-1 or PI3, and no Pestivirus antigen was detected. PCR results were positive for PPRV (7.8%), FMDV (11%), BTV (3%), OvHV-2 (31%) and BHV-1 (1.5%). None of the samples were positive for Pestiviruses.
在伊朗的13个不同地区对自由放养的野生反刍动物中的特定病毒进行了分子和血清学调查。在伊朗全国野生动物疾病调查期间，从属于四个不同物种的64只小型野生反刍动物中采集了样本，包括25只摩弗伦羊（东方盘羊）、22只野山羊（野山羊）、9只印度瞪羚（印度瞪羚）和8只鹅喉羚（鹅喉羚）。血清样本通过针对小反刍兽疫病毒（PPRV）、瘟病毒[边界病病毒（BVD）和牛病毒性腹泻病毒（BVDV）]、蓝舌病毒（BTV）、牛疱疹病毒1型（BHV-1）和副流感3型（PI3）的血清学抗体检测进行评估。血清还进行了瘟病毒抗原的ELISA检测。使用聚合酶链反应（PCR）对包括脾脏、肝脏、肺、扁桃体、肠系膜和纵隔淋巴结以及白细胞（WBC）在内的组织样本进行PPRV、口蹄疫病毒（FMDV）、瘟病毒、BTV、绵羊疱疹病毒2型（OvHV-2）和BHV-1检测。血清学检测显示，针对PPRV（17%）、瘟病毒（2%）和BTV（2%）的抗体呈阳性。未检测到针对BHV-1或PI3的抗体，也未检测到瘟病毒抗原。PCR结果显示，PPRV（7.8%）、FMDV（11%）、BTV（3%）、OvHV-2（31%）和BHV-1（1.5%）呈阳性。没有样本对瘟病毒呈阳性。
