uvrD 基因的突变会在大肠杆菌中诱导 SOS 反应。
Mutations in uvrD induce the SOS response in Escherichia coli.
作者信息
Ossanna N, Mount D W
机构信息
Department of Molecular and Cellular Biology, University of Arizona, Tucson 85721.
出版信息
J Bacteriol. 1989 Jan;171(1):303-7. doi: 10.1128/jb.171.1.303-307.1989.
Abstract
We have isolated three new mutations in uvrD that increase expression of the Escherichia coli SOS response in the absence of DNA damage. Like other uvrD (DNA helicase II) mutants, these strains are sensitive to UV irradiation and have high spontaneous mutation frequencies. Complementation studies with uvrD+ showed that UV sensitivity and spontaneous mutator activity were recessive in these new mutants. The SOS-induction phenotype, however, was not completely complemented, which indicated that the mutant proteins were functioning in some capacity. The viability of one of the mutants in combination with rep-5 suggests that the protein is functional in DNA replication. We suggest that these mutant proteins are deficient in DNA repair activities (since UV sensitivity is complemented) but are able to participate in DNA replication. We believe that defective DNA replication in these mutants increases SOS expression.
摘要
我们分离出了uvrD中的三个新突变，这些突变在没有DNA损伤的情况下会增加大肠杆菌SOS反应的表达。与其他uvrD（DNA解旋酶II）突变体一样，这些菌株对紫外线照射敏感，且自发突变频率很高。用uvrD+进行的互补研究表明，紫外线敏感性和自发诱变活性在这些新突变体中是隐性的。然而，SOS诱导表型并未完全互补，这表明突变蛋白在某种程度上仍在发挥作用。其中一个突变体与rep-5组合后的生存能力表明该蛋白在DNA复制中具有功能。我们认为这些突变蛋白在DNA修复活性方面存在缺陷（因为紫外线敏感性得到了互补），但能够参与DNA复制。我们相信这些突变体中存在缺陷的DNA复制会增加SOS的表达。
相似文献
1
Mutations in uvrD induce the SOS response in Escherichia coli.uvrD 基因的突变会在大肠杆菌中诱导 SOS 反应。
J Bacteriol. 1989 Jan;171(1):303-7. doi: 10.1128/jb.171.1.303-307.1989.
2
lon incompatibility associated with mutations causing SOS induction: null uvrD alleles induce an SOS response in Escherichia coli.与导致SOS诱导的突变相关的lon不相容性：uvrD无效等位基因在大肠杆菌中诱导SOS反应。
J Bacteriol. 2000 Jun;182(11):3151-7. doi: 10.1128/JB.182.11.3151-3157.2000.
3
Differential suppressor effects of the ssb-1 and ssb-113 alleles on uvrD mutator of Escherichia coli in DNA repair and mutagenesis.ssb-1和ssb-113等位基因对大肠杆菌uvrD突变体在DNA修复和诱变中的差异抑制作用。
J Basic Microbiol. 1987;27(5):263-73. doi: 10.1002/jobm.3620270508.
4
DNA helicases in recombination and repair: construction of a delta uvrD delta helD delta recQ mutant deficient in recombination and repair.参与重组与修复的DNA解旋酶：构建在重组与修复方面存在缺陷的ΔuvrDΔhelDΔrecQ突变体
J Bacteriol. 1995 Mar;177(5):1326-35. doi: 10.1128/jb.177.5.1326-1335.1995.
5
uvrD mutations enhance tandem repeat deletion in the Escherichia coli chromosome via SOS induction of the RecF recombination pathway.uvrD突变通过SOS诱导RecF重组途径增强大肠杆菌染色体中的串联重复序列缺失。
Mol Microbiol. 1997 Nov;26(3):557-67. doi: 10.1046/j.1365-2958.1997.6011973.x.
6
Construction and analysis of deletions in the structural gene (uvrD) for DNA helicase II of Escherichia coli.大肠杆菌DNA解旋酶II结构基因（uvrD）缺失的构建与分析
J Bacteriol. 1991 Apr;173(8):2569-75. doi: 10.1128/jb.173.8.2569-2575.1991.
7
The SOS-dependent upregulation of uvrD is not required for efficient nucleotide excision repair of ultraviolet light induced DNA photoproducts in Escherichia coli.在大肠杆菌中，uvrD的SOS依赖性上调对于紫外线诱导的DNA光产物的高效核苷酸切除修复不是必需的。
Mutat Res. 2001 May 10;485(4):319-29. doi: 10.1016/s0921-8777(01)00068-4.
8
Double helicase II (uvrD)-helicase IV (helD) deletion mutants are defective in the recombination pathways of Escherichia coli.双解旋酶II（uvrD）-解旋酶IV（helD）缺失突变体在大肠杆菌的重组途径中存在缺陷。
J Bacteriol. 1993 Aug;175(15):4641-51. doi: 10.1128/jb.175.15.4641-4651.1993.
9
Mutations in motif II of Escherichia coli DNA helicase II render the enzyme nonfunctional in both mismatch repair and excision repair with differential effects on the unwinding reaction.大肠杆菌DNA解旋酶II基序II中的突变使该酶在错配修复和切除修复中均失去功能，对解旋反应有不同影响。
J Bacteriol. 1995 Oct;177(19):5612-21. doi: 10.1128/jb.177.19.5612-5621.1995.
10
Escherichia coli uvrD mutants with thermosensitive DNA-dependent adenosine triphosphatase I (helicase II).
Mol Gen Genet. 1983;192(3):378-85. doi: 10.1007/BF00392178.
引用本文的文献
1
Comparative genomics hints at dispensability of multiple essential genes in two Escherichia coli L-form strains.比较基因组学提示在两种大肠杆菌 L 型菌株中有多个必需基因的非必需性。
Biosci Rep. 2023 Oct 31;43(10). doi: 10.1042/BSR20231227.
2
Synthetic lethal mutants in define pathways necessary for survival with RNase H deficiency.在中鉴定出的合成致死突变体，定义了 RNASE H 缺陷时生存所必需的途径。
J Bacteriol. 2023 Oct 26;205(10):e0028023. doi: 10.1128/jb.00280-23. Epub 2023 Oct 11.
3
A genetic screen to identify factors affected by undecaprenyl phosphate recycling uncovers novel connections to morphogenesis in Escherichia coli.一个遗传筛选实验，旨在识别受十一烯基磷酸循环影响的因素，揭示了大肠杆菌形态发生过程中的新的关联。
Mol Microbiol. 2021 Feb;115(2):191-207. doi: 10.1111/mmi.14609. Epub 2020 Oct 12.
4
RNase HII Saves rnhA Mutant Escherichia coli from R-Loop-Associated Chromosomal Fragmentation.核糖核酸酶HII拯救rnhA突变型大肠杆菌免受R环相关的染色体片段化影响。
J Mol Biol. 2017 Sep 15;429(19):2873-2894. doi: 10.1016/j.jmb.2017.08.004. Epub 2017 Aug 15.
5
Single Strand Annealing Plays a Major Role in RecA-Independent Recombination between Repeated Sequences in the Radioresistant Deinococcus radiodurans Bacterium.单链退火在耐辐射奇异球菌中重复序列间的RecA非依赖性重组中起主要作用。
PLoS Genet. 2015 Oct 30;11(10):e1005636. doi: 10.1371/journal.pgen.1005636. eCollection 2015 Oct.
6
The SOS Regulatory Network.SOS调控网络。
EcoSal Plus. 2008 Jul 25;2008. doi: 10.1128/ecosalplus.5.4.3.
7
Replication fork inhibition in seqA mutants of Escherichia coli triggers replication fork breakage.大肠杆菌seqA突变体中的复制叉抑制会引发复制叉断裂。
Mol Microbiol. 2014 Jul;93(1):50-64. doi: 10.1111/mmi.12638. Epub 2014 May 23.
8
The CJIE1 prophage of Campylobacter jejuni affects protein expression in growth media with and without bile salts.空肠弯曲菌的CJIE1前噬菌体在有和没有胆盐的生长培养基中影响蛋白质表达。
BMC Microbiol. 2014 Mar 19;14:70. doi: 10.1186/1471-2180-14-70.
9
UvrD Participation in Nucleotide Excision Repair Is Required for the Recovery of DNA Synthesis following UV-Induced Damage in Escherichia coli.UvrD参与核苷酸切除修复是大肠杆菌紫外线诱导损伤后DNA合成恢复所必需的。
J Nucleic Acids. 2012;2012:271453. doi: 10.1155/2012/271453. Epub 2012 Sep 27.
10
Escherichia coli Rep DNA helicase and error-prone DNA polymerase IV interact physically and functionally.大肠杆菌 Rep DNA 解旋酶和易错 DNA 聚合酶 IV 相互作用，具有物理和功能联系。
Mol Microbiol. 2011 Apr;80(2):524-41. doi: 10.1111/j.1365-2958.2011.07590.x. Epub 2011 Mar 7.
本文引用的文献
1
Complementation studies with the repair-deficient uvrD3, uvrE156, and recL152 mutations in Escherichia coli.在大肠杆菌中对修复缺陷型uvrD3、uvrE156和recL152突变进行的互补研究。
Mol Gen Genet. 1981;184(3):526-30. doi: 10.1007/BF00352533.
2
Escherichia coli mutator mutants deficient in methylation-instructed DNA mismatch correction.缺乏甲基化指导的DNA错配修复的大肠杆菌突变体
Proc Natl Acad Sci U S A. 1980 Feb;77(2):1063-7. doi: 10.1073/pnas.77.2.1063.
3
E. coli recA protein-directed cleavage of phage lambda repressor requires polynucleotide.大肠杆菌RecA蛋白介导的噬菌体λ阻遏物切割需要多核苷酸。
Nature. 1980 Jan 3;283(5742):26-30. doi: 10.1038/283026a0.
4
The Escherichia coli uvrD gene is inducible by DNA damage.大肠杆菌uvrD基因可被DNA损伤诱导。
Mol Gen Genet. 1983;191(3):397-400. doi: 10.1007/BF00425753.
5
Linkage map of Escherichia coli K-12, edition 7.大肠杆菌K-12连锁图谱，第7版。
Microbiol Rev. 1983 Jun;47(2):180-230. doi: 10.1128/mr.47.2.180-230.1983.
6
DNA repair in Escherichia coli: identification of the uvrD gene product.大肠杆菌中的DNA修复：uvrD基因产物的鉴定。
Proc Natl Acad Sci U S A. 1982 Sep;79(18):5616-20. doi: 10.1073/pnas.79.18.5616.
7
Mu dX, a derivative of Mu d1 (lac Apr) which makes stable lacZ fusions at high temperature.Mu dX，一种Mu d1（乳糖氨苄青霉素抗性）的衍生物，能在高温下形成稳定的乳糖Z融合体。
J Bacteriol. 1983 Nov;156(2):970-4. doi: 10.1128/jb.156.2.970-974.1983.
8
The uvrD gene of E. coli encodes a DNA-dependent ATPase.大肠杆菌的uvrD基因编码一种依赖DNA的ATP酶。
Nature. 1982 Jul 1;298(5869):98-100. doi: 10.1038/298098a0.
9
Location of uvr502 mutation on the chromosome of Escherichia coli K-12.uvr502突变在大肠杆菌K-12染色体上的位置。
Mol Gen Genet. 1971;113(3):217-21. doi: 10.1007/BF00339541.
10
Studies on radiation-sensitive mutants of E. coli. I. Mutants defective in the repair synthesis.大肠杆菌辐射敏感突变体的研究。I. 修复合成缺陷的突变体
Mol Gen Genet. 1968 May 3;101(3):227-44. doi: 10.1007/BF00271625.
Zotero 插件