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J Bacteriol. 1989 Jan;171(1):577-80. doi: 10.1128/jb.171.1.577-580.1989.
2
Cloning of a gene coding for phosphotransacetylase from Escherichia coli.来自大肠杆菌的磷酸转乙酰酶编码基因的克隆。
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Overproduction of acetate kinase activates the phosphate regulon in the absence of the phoR and phoM functions in Escherichia coli.
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6
Regulation of the Bacillus subtilis acetate kinase gene by CcpA.CcpA对枯草芽孢杆菌乙酸激酶基因的调控
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9
Overproduction of acetate kinase activates the phosphate regulon in the absence of the phoR and phoM functions in Escherichia coli.
J Bacteriol. 1990 May;172(5):2245-9. doi: 10.1128/jb.172.5.2245-2249.1990.
10
Cloning, sequence analysis, and functional expression of the acetyl coenzyme A synthetase gene from Methanothrix soehngenii in Escherichia coli.索氏甲烷丝菌乙酰辅酶A合成酶基因在大肠杆菌中的克隆、序列分析及功能表达
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本文引用的文献

1
Fatty acid synthesis by enzyme preparations of Clostridium kluyveri. VI. Reactions of acyl phosphates.克氏梭菌酶制剂催化的脂肪酸合成。VI. 酰基磷酸酯的反应。
J Biol Chem. 1950 Jun;184(2):769-93.
2
Enzymic formation of adenosine triphosphate with acetyl phosphate as donor in a yeast extract.在酵母提取物中以乙酰磷酸作为供体通过酶促反应形成三磷酸腺苷。
Biochim Biophys Acta. 1963 Jan 8;67:168-70.
3
Glutamine phosphoribosylpyrophosphate amidotransferase from cloned Escherichia coli purF. NH2-terminal amino acid sequence, identification of the glutamine site, and trace metal analysis.来自克隆的大肠杆菌purF的谷氨酰胺磷酸核糖焦磷酸酰胺转移酶。氨基末端氨基酸序列、谷氨酰胺位点的鉴定及痕量金属分析。
J Biol Chem. 1982 Apr 10;257(7):3532-6.
4
Galactose fermentation by Streptococcus lactis and Streptococcus cremoris: pathways, products, and regulation.乳酸链球菌和乳脂链球菌的半乳糖发酵：途径、产物及调控
J Bacteriol. 1980 Nov;144(2):672-82. doi: 10.1128/jb.144.2.672-682.1980.
5
Specific mistranslation in hisT mutants of Escherichia coli.大肠杆菌hisT突变体中的特异性错译
Mol Gen Genet. 1982;187(3):405-9. doi: 10.1007/BF00332619.
6
Linkage map of Escherichia coli K-12, edition 7.大肠杆菌K-12连锁图谱，第7版。
Microbiol Rev. 1983 Jun;47(2):180-230. doi: 10.1128/mr.47.2.180-230.1983.
7
Lambda replacement vectors carrying polylinker sequences.携带多克隆位点序列的λ置换载体。
J Mol Biol. 1983 Nov 15;170(4):827-42. doi: 10.1016/s0022-2836(83)80190-9.
8
New M13 vectors for cloning.用于克隆的新型M13载体。
Methods Enzymol. 1983;101:20-78. doi: 10.1016/0076-6879(83)01005-8.
9
Nucleotide sequence of Escherichia coli purF and deduced amino acid sequence of glutamine phosphoribosylpyrophosphate amidotransferase.大肠杆菌嘌呤F的核苷酸序列及谷氨酰胺磷酸核糖焦磷酸酰胺转移酶的推导氨基酸序列。
J Biol Chem. 1982 Apr 10;257(7):3525-31.
10
Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.利用单链噬菌体克隆辅助快速DNA测序。
J Mol Biol. 1980 Oct 25;143(2):161-78. doi: 10.1016/0022-2836(80)90196-5.

大肠杆菌K-12 ackA基因的克隆、表达及核苷酸序列

Cloning, expression, and nucleotide sequence of the Escherichia coli K-12 ackA gene.

作者信息

Matsuyama A, Yamamoto H, Nakano E

机构信息

Research and Development Division, Kikkoman Corporation, Chiba-ken, Japan.

出版信息

J Bacteriol. 1989 Jan;171(1):577-80. doi: 10.1128/jb.171.1.577-580.1989.

DOI:10.1128/jb.171.1.577-580.1989
PMID:2536666
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC209626/
Abstract

The Escherichia coli K-12 ackA gene, which encodes an acetate kinase, was cloned. The acetate kinase activities of ackA+ plasmid-containing strains were amplified 160- to 180-fold. The complete nucleotide sequence of the ackA gene was determined. It was deduced that the ackA gene coded for a protein of 400 amino acids with an Mr of 43,297. The ackA gene was found to be located about 15 kilobases upstream of the purF-folC-hisT region of the chromosome.

摘要

编码乙酸激酶的大肠杆菌K-12 ackA基因被克隆。含有ackA+质粒的菌株的乙酸激酶活性增强了160至180倍。测定了ackA基因的完整核苷酸序列。据推断，ackA基因编码一种由400个氨基酸组成、分子量为43297的蛋白质。发现ackA基因位于染色体上purF-folC-hisT区域上游约15千碱基处。