大肠杆菌recF突变体中对烷基化损伤的适应性反应诱导改变。
Altered induction of the adaptive response to alkylation damage in Escherichia coli recF mutants.
作者信息
Volkert M R
机构信息
Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester 01655.
出版信息
J Bacteriol. 1989 Jan;171(1):99-103. doi: 10.1128/jb.171.1.99-103.1989.
Abstract
Escherichia coli recF mutants are hypermutable when treated with methyl methanesulfonate (G. C. Walker, Mol. Gen. Genet. 152:93-103, 1977). In this study, methylation hypermutability of recF mutant strains was examined, and it was found that recF+ is required for normal induction of the adaptive response to alkylation damage. Although this regulatory effect of recF mutations results in reduced levels of enzymes that specifically repair methyl lesions in DNA, it only partially explains the hypermutability. Further examination showed that methylation hypermutability of recF mutant strains required a functional umuDC operon, a component of the SOS response. These results lead to the hypothesis that methylation hypermutability results from the effects of recF mutations on the induction of both the SOS response and the adaptive response.
摘要
用甲磺酸甲酯处理时，大肠杆菌recF突变体具有高度突变性（G.C.沃克，《分子与普通遗传学》152:93 - 103，1977）。在本研究中，对recF突变体菌株的甲基化高度突变性进行了检测，发现recF +对于正常诱导对烷基化损伤的适应性反应是必需的。尽管recF突变的这种调节作用导致特异性修复DNA中甲基损伤的酶水平降低，但这只是部分解释了高度突变性。进一步检测表明，recF突变体菌株的甲基化高度突变性需要功能性的umuDC操纵子，这是SOS反应的一个组成部分。这些结果引出一个假说，即甲基化高度突变性是recF突变对SOS反应和适应性反应诱导的影响所致。
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