构建用于通过发光检测诱变DNA修复的umuC'-luxAB质粒。
Construction of a umuC'-luxAB plasmid for the detection of mutagenic DNA repair via luminescence.
作者信息
Justus T, Thomas S M
机构信息
School of Biological Sciences, The Flinders University of South Australia, Adelaide, Australia.
出版信息
Mutat Res. 1998 Feb 26;398(1-2):131-41. doi: 10.1016/s0027-5107(97)00215-7.
This paper describes a novel system for the detection of mutagenic DNA repair in Escherichia coli. The DNA damage inducible umuC gene of Escherichia coli has been fused to the luxAB genes from Vibrio harvleyi that encode the enzyme luciferase. Mutagenicity has been assessed semi-quantitatively by the induction of bioluminescence. This system is simple, rapid and equivalent in sensitivity to other currently available test procedures. Its use in the detection of known SOS mutagens MMS, MNNG and UV is described.
本文描述了一种用于检测大肠杆菌中诱变DNA修复的新型系统。大肠杆菌中受DNA损伤诱导的umuC基因已与哈氏弧菌编码荧光素酶的luxAB基因融合。通过生物发光的诱导对诱变性进行了半定量评估。该系统简单、快速，灵敏度与目前其他可用的测试程序相当。文中描述了其在检测已知的SOS诱变剂甲基磺酸甲酯（MMS）、N-甲基-N'-硝基-N-亚硝基胍（MNNG）和紫外线中的应用。
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