Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional, Unidad Oaxaca, CP. 68030, Oaxaca, México.
Food Funct. 2014 Dec;5(12):3311-9. doi: 10.1039/c4fo00561a.
Agavins act as a fermentable dietary fiber and have attracted attention due to their potential for reducing the risk of disease. Therefore, we evaluated the effect of supplementation using 10% agavins with a short-degree of polymerization (SDP) from Agave angustifolia Haw. (AASDP) or Agave potatorum Zucc. (APSDP) along with chicory fructans (RSE) as a reference for 5 weeks, on the energy intake, body weight gain, satiety-related hormones from the gut and blood (GLP-1 and ghrelin), blood glucose and lipids, and short-chain fatty acids (SCFAs) from the gut of ad libitum-fed mice. We evaluated the energy intake daily and weight gain every week. At the end of the experiment, portal vein blood samples as well as intestinal segments and the stomach were collected to measure glucagon-like peptide-1 (GLP-1) and ghrelin using RIA and ELISA kits, respectively. Colon SCFAs were measured using gas chromatography. The energy intake, body weight gain, and triglycerides were lower in the fructan-fed mice than in the STD-fed mice. The AASDP, APSDP, and RSE diets increased the serum levels of GLP-1 (40, 93, and 16%, respectively vs. STD) (P ≤ 0.05), whereas ghrelin was decreased (16, 38, and 42%, respectively) (P ≤ 0.05). Butyric acid increased significantly in the APSDP-fed mice (26.59 mmol g(-1), P ≤ 0.001) compared with that in the AASDP- and RSE-fed mice. We concluded that AASDP and APSDP are able to promote the secretion of the peptides involved in appetite regulation, which might help to control obesity and its associated metabolic disorder.
藻寡糖作为一种可发酵膳食纤维，因其具有降低疾病风险的潜力而受到关注。因此，我们评估了在 5 周时间内，使用来自龙舌兰（AASDP）或剑麻（APSDP）的具有短聚合度（SDP）的 10％藻寡糖（AASDP）或 Agave potatorum Zucc.（APSDP）与菊苣果聚糖（RSE）补充剂对随意进食小鼠的能量摄入、体重增加、肠道和血液中的饱腹感相关激素（GLP-1 和胃饥饿素）、血糖和血脂以及肠道短链脂肪酸（SCFAs）的影响。我们每天评估能量摄入，每周评估体重增加。在实验结束时，采集门静脉血液样本以及肠道段和胃，分别使用 RIA 和 ELISA 试剂盒测量胰高血糖素样肽-1（GLP-1）和胃饥饿素，使用气相色谱法测量结肠 SCFAs。与 STD 喂养的小鼠相比，果聚糖喂养的小鼠的能量摄入、体重增加和甘油三酸酯水平较低。AASDP、APSDP 和 RSE 饮食分别使血清中 GLP-1 水平增加（40、93 和 16％，分别比 STD 高）（P ≤ 0.05），而胃饥饿素降低（16、38 和 42％，分别）（P ≤ 0.05）。与 AASDP 和 RSE 喂养的小鼠相比，APSDP 喂养的小鼠的丁酸含量显著增加（26.59mmol/g-1，P ≤ 0.001）。我们得出结论，AASDP 和 APSDP 能够促进参与食欲调节的肽的分泌，这可能有助于控制肥胖及其相关的代谢紊乱。
