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腺相关病毒在254纳米紫外线照射的细胞中的复制。

Replication of adeno-associated virus in cells irradiated with UV light at 254 nm.

作者信息

Yakobson B, Hrynko T A, Peak M J, Winocour E

机构信息

Department of Virology, Weizmann Institute of Science, Rehovot, Israel.

出版信息

J Virol. 1989 Mar;63(3):1023-30. doi: 10.1128/JVI.63.3.1023-1030.1989.

DOI:10.1128/JVI.63.3.1023-1030.1989
PMID:2536816
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC247794/
Abstract

Irradiation of simian virus 40 (ori mutant)-transformed Chinese hamster embryo cells (OD4 line) with UV light induced a cellular capacity which supported a full cycle of helper-independent adeno-associated virus replication. Monochromatic UV light at 254 nm was about 1,000-fold more effective than UV light at 313 nm, indicating that cellular nucleic acid is the primary chromophore in the UV-induced process leading to permissiveness for adeno-associated virus replication. The UV irradiation and the infection could be separated for up to 12 h without substantial loss of permissiveness. During this time interval, the induction process was partly sensitive to cycloheximide, suggesting a requirement for de novo protein synthesis.

摘要

用紫外线照射猿猴病毒40（ori突变体）转化的中国仓鼠胚胎细胞（OD4系）可诱导细胞产生一种能力，这种能力支持无辅助腺相关病毒的完整复制周期。254 nm的单色紫外线比313 nm的紫外线有效约1000倍，这表明细胞核酸是紫外线诱导过程中导致腺相关病毒复制许可的主要发色团。紫外线照射和感染可相隔长达12小时，而许可性不会有实质性损失。在此时间间隔内，诱导过程对放线菌酮部分敏感，提示需要从头合成蛋白质。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f729/247794/df0d7cf416ce/jvirol00070-0013-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f729/247794/df0d7cf416ce/jvirol00070-0013-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f729/247794/df0d7cf416ce/jvirol00070-0013-a.jpg

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本文引用的文献

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Science. 1950 Mar 3;111(2879):229. doi: 10.1126/science.111.2879.229-a.
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Ultraviolet light action spectra for neoplastic transformation and lethality of Syrian hamster embryo cells correlate with spectrum for pyrimidine dimer formation in cellular DNA.叙利亚仓鼠胚胎细胞发生肿瘤转化和致死的紫外线作用光谱与细胞DNA中嘧啶二聚体形成的光谱相关。
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