Cherrington J M, Mocarski E S
Department of Microbiology and Immunology, Stanford University School of Medicine, California 94305.
J Virol. 1989 Mar;63(3):1435-40. doi: 10.1128/JVI.63.3.1435-1440.1989.
The expression of alpha (immediate-early) genes of cytomegalovirus is regulated via a complex enhancer that consists of several different repeat elements. We describe here the autoinduction of expression from the alpha promoter-enhancer by the most abundant alpha gene product, a 491-amino-acid nuclear phosphoprotein referred to as ie1. We defined the 18-base-pair repeat element within the alpha enhancer as the signal through which ie1 acts to regulate gene expression. This element contains an NF kappa B site that may play an important role in ie1 autoregulation. Our analysis, which relied on deletions through the enhancer as well as reconstitution of responsiveness to a promoter with synthetic 18-base-pair repeats, strongly implicated ie1 in the transcriptional transactivation of the alpha promoter through its enhancer.
巨细胞病毒α（即刻早期）基因的表达是通过一个由几种不同重复元件组成的复杂增强子来调控的。我们在此描述了最丰富的α基因产物，一种被称为ie1的491个氨基酸的核磷蛋白对α启动子-增强子表达的自诱导作用。我们将α增强子内18个碱基对的重复元件定义为ie1作用以调控基因表达的信号。该元件包含一个可能在ie1自调控中起重要作用的NF-κB位点。我们的分析依赖于通过增强子的缺失以及用合成的18个碱基对重复序列对启动子反应性的重建，有力地表明ie1通过其增强子对α启动子进行转录反式激活。
