Thomsen D R, Stenberg R M, Goins W F, Stinski M F
Proc Natl Acad Sci U S A. 1984 Feb;81(3):659-63. doi: 10.1073/pnas.81.3.659.
The DNA templates containing immediate early (IE) genes of human cytomegalovirus (CMV) were transcribed in vitro by using a HeLa cell extract. When IE region 1, 2, and 3 were used, transcription was detected qualitatively only from IE region 1. Transcription was detected with DNA representing IE region 2 when the IE region 1 promoter was not present. DNA sequence analysis of the upstream regulatory region of IE region 1 detected two distinct repeats of 19 and 18 nucleotides, both being repeated four times. A putative cruciform structure could form through the surrounding sequences with each 18-nucleotide repeat being located in the unpaired region. The potential secondary structure and the repeat sequences in the regulatory region of IE region 1 are presumably related to the high level of transcription of this IE gene.
使用HeLa细胞提取物在体外转录包含人巨细胞病毒（CMV）即刻早期（IE）基因的DNA模板。当使用IE区域1、2和3时，仅从IE区域1定性检测到转录。当不存在IE区域1启动子时，用代表IE区域2的DNA检测到转录。对IE区域1上游调控区域的DNA序列分析检测到19和18个核苷酸的两个不同重复序列，两者均重复四次。通过周围序列可以形成一个假定的十字形结构，每个18个核苷酸的重复序列位于未配对区域。IE区域1调控区域中的潜在二级结构和重复序列可能与该IE基因的高水平转录有关。
