荷叶黄酮对四氯化碳诱导的小鼠肝损伤的改善作用

Improvement Effect of Lotus Leaf Flavonoids on Carbon Tetrachloride-Induced Liver Injury in Mice.

作者信息

Liu Tongji, Tan Fang, Long Xingyao, Pan Yanni, Mu Jianfei, Zhou Xianrong, Yi Runkun, Zhao Xin

机构信息

Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, China.

Chongqing Engineering Research Center of Functional Food, Chongqing University of Education, Chongqing 400067, China.

出版信息

Biomedicines. 2020 Feb 24;8(2):41. doi: 10.3390/biomedicines8020041.

DOI:10.3390/biomedicines8020041

PMID:32102401

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7169453/

Abstract

In this study, the effect of lotus leaf flavonoids (LLF) on carbon tetrachloride (CCl)-induced liver injury in mice was studied. CCl was injected intraperitoneally to induce liver injury in Kunming mice. Mice were treated with LLF by gavage, and the mRNA expression levels in serum and liver were detected. Compared with the model group, LLF significantly reduced the liver index and serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), triglyceride (TG), and total cholesterol (TC) levels in mice with CCl-induced liver injury. Pathological observation showed that LLF effectively reduced morphological incompleteness and hepatocyte necrosis in CCl-treated liver tissue. The result of quantitative polymerase chain reaction (qPCR) indicated that LLF significantly up-regulated the mRNA expression levels of copper/zinc superoxide dismutase (Cu/Zn-SOD), manganese superoxide dismutase (Mn-SOD), and catalase (CAT) and down- regulated the expression levels of tumor necrosis factor-alpha (TNF-α), nuclear factor kappa B (NF-κB), and interleukin-1β (IL-1β) ( < 0.05). Thus, LLF is an active ingredient that ameliorates liver injury, and it has good application prospect.

摘要

本研究探讨了荷叶黄酮（LLF）对四氯化碳（CCl）诱导的小鼠肝损伤的影响。通过腹腔注射CCl诱导昆明小鼠肝损伤。采用灌胃法给予小鼠LLF处理，并检测血清和肝脏中的mRNA表达水平。与模型组相比，LLF显著降低了CCl诱导的肝损伤小鼠的肝脏指数以及血清天冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）、甘油三酯（TG）和总胆固醇（TC）水平。病理观察表明，LLF有效减轻了CCl处理的肝组织的形态不完整和肝细胞坏死。定量聚合酶链反应（qPCR）结果表明，LLF显著上调了铜/锌超氧化物歧化酶（Cu/Zn-SOD）、锰超氧化物歧化酶（Mn-SOD）和过氧化氢酶（CAT）的mRNA表达水平，并下调了肿瘤坏死因子-α（TNF-α）、核因子κB（NF-κB）和白细胞介素-1β（IL-1β）的表达水平（<0.05）。因此，LLF是一种改善肝损伤的活性成分，具有良好的应用前景。