Assouline J G, Pantazis N J
Department of Anatomy, University of Iowa Medical College, Iowa City 52242.
Brain Res Dev Brain Res. 1989 Jan 1;45(1):1-14. doi: 10.1016/0165-3806(89)90002-3.
Cultures of human Schwann cells and astrocytes were established from fetal nerves and brains respectively. The human Schwann cells in culture expressed a nerve growth factor (NGF) receptor as determined by indirect immunofluorescence, autoradiography, and immunoprecipitation. In contrast, the human astrocytes in culture did not have an NGF receptor. Cultures of rat Schwann cells and astrocytes were also established for comparison, with similar results. The rat Schwann cells had an NGF receptor whereas the astrocytes did not. The functional significance of this NGF receptor on Schwann cells, as well as the lack of the receptor on astrocytes, is discussed.
人雪旺细胞和星形胶质细胞的培养物分别从胎儿神经和大脑中建立。通过间接免疫荧光、放射自显影和免疫沉淀测定，培养的人雪旺细胞表达神经生长因子（NGF）受体。相比之下，培养的人星形胶质细胞没有NGF受体。还建立了大鼠雪旺细胞和星形胶质细胞的培养物用于比较，结果相似。大鼠雪旺细胞有NGF受体，而星形胶质细胞没有。本文讨论了雪旺细胞上这种NGF受体的功能意义以及星形胶质细胞上缺乏该受体的情况。
