Scarpini E, Ross A H, Rosen J L, Brown M J, Rostami A, Koprowski H, Lisak R P
Department of Neurology, Dino Ferrari Center for Neuromuscular Diseases, University of Milan, Italy.
Dev Biol. 1988 Feb;125(2):301-10. doi: 10.1016/0012-1606(88)90213-8.
The expression of NGF receptors on human Schwann cells during development and myelination and in culture was analyzed using a murine monoclonal antibody to human NGF receptor. Nonmyelinated femoral nerves from 13- to 14-week fetuses stained strongly for NGF receptor, whereas tissues from later stages of development showed a decrease in the staining intensity. These changes correlated with the initiation of myelination (17-19 weeks), as observed by phase-contrast and electron microscopy, and the reactivity with monoclonal antibody 4C5, a marker of mature Schwann cells. In adult nerves, only the perineurium and few endoneurial cells were stained with anti-NGF receptor antibody. Cultured human fetal Schwann cells were positive for NGF receptor by immunofluorescence irregardless of donor age or length of time in culture. The decreased staining of NGF receptor with nerve maturation may reflect a dependence of antigen expression on Schwann cell differentiation and/or neuron-Schwann cell interaction.
利用抗人神经生长因子（NGF）受体的鼠单克隆抗体，分析了人雪旺细胞在发育、髓鞘形成过程中以及在培养条件下NGF受体的表达情况。13至14周胎儿的无髓鞘股神经对NGF受体染色强烈，而发育后期的组织染色强度降低。这些变化与髓鞘形成的起始阶段（17至19周）相关，这是通过相差显微镜和电子显微镜观察到的，并且与成熟雪旺细胞的标志物单克隆抗体4C5的反应性相关。在成人神经中，只有神经束膜和少数神经内膜细胞被抗NGF受体抗体染色。培养的人胎儿雪旺细胞通过免疫荧光检测显示NGF受体呈阳性，与供体年龄或培养时间长短无关。随着神经成熟，NGF受体染色的减少可能反映了抗原表达对雪旺细胞分化和/或神经元 - 雪旺细胞相互作用的依赖性。
