Gavaret J M, Matricon C, Pomerance M, Jacquemin C, Toru-Delbauffe D, Pierre M
I.N.S.E.R.M. U96, Unité de Recherche sur la Glande Thyroide et la Régulation Hormonale, Le Kremlin-Bicêtre, France.
Brain Res Dev Brain Res. 1989 Jan 1;45(1):77-82. doi: 10.1016/0165-3806(89)90009-6.
Basic (b) and acidic (a) forms of the fibroblast growth factor (FGF) promoted a rapid increase of the cytosolic S6 kinase activity in astroglial cells. S6 kinase activity was maximal 10 min after addition of the factors to cell cultures and remained at this level for at least 30 min. Half-activation of the enzyme was obtained with 3 ng/ml FGFa. Heparin (100 micrograms/ml) potentiated the response to suboptimal concentrations of FGFa. This growth factor appeared to stimulate an astroglial S6 kinase resembling that stimulated by insulin, IGF1, TPA and cAMP. Although FGFb is more potent than FGFa in stimulating proliferation of Chinese hamster lung fibroblasts (CCL39), it was not more efficient than FGFa in stimulating the S6 kinase activity of astroglial cells.
成纤维细胞生长因子（FGF）的碱性（b）和酸性（a）形式可促进星形胶质细胞中细胞溶质S6激酶活性的快速增加。将这些因子添加到细胞培养物中10分钟后，S6激酶活性达到最大值，并至少保持30分钟。3 ng/ml的FGFa可使该酶达到半激活状态。肝素（100微克/毫升）可增强对次优浓度FGFa的反应。这种生长因子似乎刺激了一种类似于胰岛素、IGF1、TPA和cAMP所刺激的星形胶质细胞S6激酶。尽管FGFb在刺激中国仓鼠肺成纤维细胞（CCL39）增殖方面比FGFa更有效，但在刺激星形胶质细胞的S6激酶活性方面，它并不比FGFa更有效。
