Kiyabu M T, Shibata D, Arnheim N, Martin W J, Fitzgibbons P L
Department of Pathology, Los Angeles County-University of Southern California Medical Center 90033.
Am J Surg Pathol. 1989 Mar;13(3):221-4. doi: 10.1097/00000478-198903000-00007.
We analyzed 88 formalin-fixed, paraffin-embedded invasive squamous carcinomas for human papillomavirus-related DNA sequences (HPV types 16 and 18) following in vitro gene amplification using the polymerase chain reaction. HPV DNA sequences were found in 35 of 50 (70%) carcinomas of the anogenital region, including four of four (100%) anal, six of eight (75%) vulvar, nine of 14 (64%) vaginal, two of five (40%) penile, and 14 of 19 (74%) cervical tumors. Nine of 25 (36%) oropharyngeal squamous carcinomas contained HPV DNA sequences, including four of 10 (40%) laryngeal, three of eight (38%) buccal, and two of seven (29%) glossal tumors. HPV DNA sequences were not found in 13 esophageal carcinomas. Of the 44 cases that contained viral DNA, HPV-16 was detected in 41 cases (93%) and HPV-18 in five cases (11%), while both types were found in two cases (one anal and one vulvar). HPV DNA sequences were found in 43 of 83 (52%) nonverrucous and in one of five (20%) verrucous carcinomas, but this difference was not significant. These findings demonstrate that HPV DNA sequences are more frequently associated with anogenital than oropharyngeal squamous carcinomas and can be readily detected in routinely processed tissues using the polymerase chain reaction.
我们使用聚合酶链反应进行体外基因扩增后，对88例福尔马林固定、石蜡包埋的浸润性鳞状癌进行了人乳头瘤病毒相关DNA序列（16型和18型人乳头瘤病毒）分析。在50例（70%）肛门生殖器区域癌中的35例中发现了人乳头瘤病毒DNA序列，其中包括4例（100%）肛管癌中的4例、8例（75%）外阴癌中的6例、14例（64%）阴道癌中的9例、5例（40%）阴茎癌中的2例以及19例（74%）宫颈癌中的14例。25例（36%）口咽鳞状癌中的9例含有人乳头瘤病毒DNA序列，其中包括10例（40%）喉癌中的4例、8例（38%）颊癌中的3例以及7例（29%）舌癌中的2例。13例食管癌中未发现人乳头瘤病毒DNA序列。在44例含有病毒DNA的病例中，41例（93%）检测到16型人乳头瘤病毒，5例（11%）检测到18型人乳头瘤病毒，2例（1例肛管癌和1例外阴癌）同时发现两种类型。在83例（52%）非疣状癌中的43例以及5例（20%）疣状癌中的1例发现了人乳头瘤病毒DNA序列，但这种差异不显著。这些发现表明，人乳头瘤病毒DNA序列与肛门生殖器鳞状癌的相关性比与口咽鳞状癌的相关性更频繁，并且使用聚合酶链反应可在常规处理的组织中轻易检测到。
