College of Life Sciences, Shandong Province Key Laboratory of Applied Mycology, Qingdao Agricultural University, Qingdao, 266109, China.
College of Agronomy and Plant Protection, the Key Laboratory of Integrated Crop Pest Management of Shandong Province, Qingdao Agricultural University, No. 700 Changcheng Road, Qingdao, 266109, China.
Appl Biochem Biotechnol. 2018 Jan;184(1):168-181. doi: 10.1007/s12010-017-2538-9. Epub 2017 Jun 27.
Microbial oils are among the most attractive alternative feedstocks for biodiesel production. In this study, a newly isolated yeast strain, AM113 of Papiliotrema laurentii, was identified as a potential lipid producer, which could accumulate a large amount of intracellular lipids from hydrolysates of inulin. P. laurentii AM113 was able to produce 54.6% (w/w) of intracellular oil in its cells and 18.2 g/l of dry cell mass in a fed-batch fermentation. The yields of lipid and biomass were 0.14 and 0.25 g per gram of consumed sugar, respectively. The lipid productivity was 0.092 g of oil per hour. Compositions of the fatty acids produced were C (0.9%), C (10.8%), C (9.7%), C (6.5%), C (60.3%), and C (11.8%). Biodiesel obtained from the extracted lipids could be burnt well. This study not only provides a promising candidate for single cell oil production, but will also probably facilitate more efficient biodiesel production.
微生物油脂是最有吸引力的生物柴油生产替代原料之一。在本研究中，从菊粉水解物中积累大量细胞内油脂的新分离酵母菌株，Papiliotrema laurentii 的 AM113 被鉴定为一种有潜力的产脂生物，它可以在分批补料发酵中产生 54.6%（w/w）的细胞内油和 18.2 g/l 的干细胞质量。脂质和生物质的产率分别为每消耗 1 克糖产生 0.14 和 0.25 克。脂质的生产力为每小时 0.092 克油。产生的脂肪酸组成分别为 C（0.9%）、C（10.8%）、C（9.7%）、C（6.5%）、C（60.3%）和 C（11.8%）。从提取的油脂中获得的生物柴油可以很好地燃烧。本研究不仅为单细胞油生产提供了一个有前途的候选者，而且可能还会促进更高效的生物柴油生产。
