Arosio Daniela, Chiodo Fabrizio, Reina José J, Marelli Marcello, Penadés Soledad, van Kooyk Yvette, Garcia-Vallejo Juan J, Bernardi Anna
CNR-Institute of Molecular Science and Technologies (ISTM) , via Golgi 19, I-20133 Milan, Italy.
Bioconjug Chem. 2014 Dec 17;25(12):2244-51. doi: 10.1021/bc500467u. Epub 2014 Nov 24.
Dendritic Cells (DCs), the most potent antigen-presenting cells, play a critical role in the detection of invading pathogens, which are recognized also by multiple carbohydrate-specific receptors. Among them, DC-SIGN is one of the best characterized, with high-mannose and Lewis-type glycan specificity. In this study, we present a potent DC-SIGN targeting device developed using gold nanoparticles functionalized with α-fucosyl-β-alanyl amide. The nanoparticles bound to cellular DC-SIGN and induced internalization as effectively as similar particles coated with comparable amounts of Lewis(X) oligosaccharide. They were found to be neutral toward dendritic cell maturation and IL-10 production, thus envisaging a possible use as targeted imaging tools and antigen delivery devices.
树突状细胞（DCs）是最有效的抗原呈递细胞，在检测入侵病原体中起关键作用，这些病原体也被多种碳水化合物特异性受体识别。其中，DC-SIGN是特征最明确的受体之一，具有高甘露糖和Lewis型聚糖特异性。在本研究中，我们展示了一种使用用α-岩藻糖基-β-丙氨酸酰胺功能化的金纳米颗粒开发的高效DC-SIGN靶向装置。这些纳米颗粒与细胞DC-SIGN结合，并与涂覆有相当量Lewis(X)寡糖的类似颗粒一样有效地诱导内化。发现它们对树突状细胞成熟和IL-10产生呈中性，因此设想其可能用作靶向成像工具和抗原递送装置。
