Schuurman H J, Schemmann M H, de Weger R A, Aanstoot H, Hene R
Department of Internal Medicine, University Hospital, Utrecht, The Netherlands.
Am J Clin Pathol. 1989 Apr;91(4):461-3. doi: 10.1093/ajcp/91.4.461.
The proposed role of Epstein-Barr virus (EBV) in salivary gland destruction in Sjögren's syndrome (SS) prompted the authors to study the presence of EBV-DNA (hybridohistochemistry) and EBV-encoded proteins (immunohistochemistry) in sublabial salivary glands taken from eight patients with primary and five with secondary SS and from 16 controls. DNA probes and anti-EBV antibodies were controlled for activity by assessment of human blood B-lymphocytes after in vitro infection with EBV. None of the tissues investigated manifested the presence of EBV proteins (nuclear antigen, early antigen R, membrane antigen, or viral capsid antigen). The salivary gland biopsies of four patients with primary SS and two with secondary SS showed EBV-DNA in epithelial cells of acini and ducts but not in other components. The authors data contrast with those of Fox and colleagues (J Immunol 1986;137:3162-3168), who reported that about half of the patients with SS have EBV early antigen D in epithelium of the sublabial salivary gland. The authors conclude that an active EBV infection associated with EBV protein synthesis does not occur in the diseased salivary gland of patients with SS, but the presence of EBV-DNA in the glands does not exclude a possible role of EBV in the disorder.
爱泼斯坦-巴尔病毒（EBV）在干燥综合征（SS）唾液腺破坏中可能发挥的作用，促使作者研究了取自8例原发性SS患者、5例继发性SS患者以及16例对照者的唇下唾液腺中EBV-DNA（杂交组织化学）和EBV编码蛋白（免疫组织化学）的存在情况。通过评估体外感染EBV后的人血B淋巴细胞来控制DNA探针和抗EBV抗体的活性。所研究的组织均未显示EBV蛋白（核抗原、早期抗原R、膜抗原或病毒衣壳抗原）的存在。4例原发性SS患者和2例继发性SS患者的唾液腺活检显示，腺泡和导管的上皮细胞中有EBV-DNA，但其他成分中没有。作者的数据与Fox及其同事（《免疫学杂志》1986年；137：3162 - 3168）的数据形成对比，后者报告约一半的SS患者唇下唾液腺上皮中有EBV早期抗原D。作者得出结论，SS患者患病的唾液腺中未发生与EBV蛋白合成相关的活跃EBV感染，但腺体中EBV-DNA的存在并不排除EBV在该疾病中可能发挥的作用。
