Hommes R W, Herman P T, Postma P W, Tempest D W, Neijssel O M
Department of Microbiology, University of Amsterdam, The Netherlands.
Arch Microbiol. 1989;151(3):257-60. doi: 10.1007/BF00413139.
No holoenzyme pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase and only very low apoenzyme levels could be detected in cells of Klebsiella pneumoniae, growing anaerobically, or carrying out a fumarate or nitrate respiration. Low glucose dehydrogenase activity in some aerobic glucose-excess cultures of K. pneumoniae (ammonia or sulphate limitation) was increased significantly by addition of PQQ, whereas in cells already possessing a high glucose dehydrogenase activity (phosphate or potassium limitation) extra PQQ had almost no effect. These observations indicate that the glucose dehydrogenase activity in K. pneumoniae is modulated by both PQQ synthesis and synthesis of the glucose dehydrogenase apo-enzyme.
在厌氧生长、进行富马酸盐或硝酸盐呼吸的肺炎克雷伯菌细胞中，未检测到全酶吡咯喹啉醌（PQQ）依赖性葡萄糖脱氢酶，仅检测到极低水平的脱辅基酶。在一些肺炎克雷伯菌的好氧高糖培养物（氨或硫酸盐限制）中，低葡萄糖脱氢酶活性通过添加PQQ显著增加，而在已经具有高葡萄糖脱氢酶活性的细胞（磷酸盐或钾限制）中，额外添加PQQ几乎没有效果。这些观察结果表明，肺炎克雷伯菌中的葡萄糖脱氢酶活性受PQQ合成和葡萄糖脱氢酶脱辅基酶合成的调节。
