Schnee Christiane, Sachse Konrad
Friedrich-Loeffler-Institut (Federal Research Institute for Animal Health), Naumburger Str. 96a, Jena, 07743, Germany.
Methods Mol Biol. 2015;1247:193-208. doi: 10.1007/978-1-4939-2004-4_15.
Rapid detection of slow-growing or non-culturable microorganisms, such as Mycoplasma spp. and Chlamydia spp., is still a challenge to diagnosticians in the veterinary field. In addition, as epidemiological evidence on the frequency of mixed infections involving two and more bacterial species has been emerging, detection methods allowing simultaneous identification of different pathogens are required. In the present chapter, we describe DNA microarray-based procedures for the detection of 83 Mollicutes species (Mycoplasma assay) and 11 Chlamydia spp. (Chlamydia assay). The assays are suitable for use in a routine diagnostic environment, as well as in microbiological research.
快速检测生长缓慢或不可培养的微生物，如支原体属和衣原体属，对兽医领域的诊断人员来说仍是一项挑战。此外，随着涉及两种及更多细菌种类的混合感染频率的流行病学证据不断出现，需要能够同时鉴定不同病原体的检测方法。在本章中，我们描述了基于DNA微阵列的程序，用于检测83种支原体（支原体检测）和11种衣原体（衣原体检测）。这些检测方法适用于常规诊断环境以及微生物学研究。
