Nilghaz Azadeh, Zhang Liyuan, Li Miaosi, Ballerini David R, Shen Wei
Department of Chemical Engineering, Monash University , Clayton Campus, Victoria 3800, Australia.
ACS Appl Mater Interfaces. 2014 Dec 24;6(24):22209-15. doi: 10.1021/am505849e. Epub 2014 Dec 2.
"Thread-based microfluidics" research has so far focused on utilizing and manipulating the wicking properties of threads to form controllable microfluidic channels. In this study we aim to understand the separation properties of threads, which are important to their microfluidic detection applications for blood analysis. Confocal microscopy was utilized to investigate the effect of the microscale surface morphologies of fibers on the thread's separation efficiency of red blood cells. We demonstrated the remarkably different separation properties of threads made using silk and cotton fibers. Thread separation properties dominate the clarity of blood typing assays of the ABO groups and some of their weak subgroups (Ax and A3). The microfluidic thread-based analytical devices (μTADs) designed in this work were used to accurately type different blood samples, including 89 normal ABO and 6 weak A subgroups. By selecting thread with the right surface morphology, we were able to build μTADs capable of providing rapid and accurate typing of the weak blood groups with high clarity.
“基于丝线的微流体技术”研究目前主要集中在利用和操控丝线的毛细作用特性来形成可控的微流体通道。在本研究中，我们旨在了解丝线的分离特性，这对其用于血液分析的微流体检测应用至关重要。利用共聚焦显微镜研究了纤维微观表面形态对丝线分离红细胞效率的影响。我们展示了由丝绸和棉纤维制成的丝线具有显著不同的分离特性。丝线的分离特性主导了ABO血型及其一些弱亚型（Ax和A3）血型鉴定试验的清晰度。本研究中设计的基于微流体丝线的分析装置（μTADs）用于准确鉴定不同的血液样本，包括89例正常ABO血型和6例弱A亚型。通过选择具有合适表面形态的丝线，我们能够构建出能够快速、准确且清晰地鉴定弱血型的μTADs。
