Oberley L W, McCormick M L, Sierra-Rivera E, Kasemset-St Clair D
Radiation Research Laboratory, University of Iowa, Iowa City 52242.
Free Radic Biol Med. 1989;6(4):379-84. doi: 10.1016/0891-5849(89)90083-x.
The manganese superoxide dismutase (MnSOD) activity of W138 human embryonic lung fibroblasts and SV40-transformed WI38 cells was measured. Under various growth conditions, the transformed cells always had lower MnSOD activity than their normal cell counterparts. The activity of MnSOD changes greatly with the growth conditions in the WI38 cells, while the MnSOD activity in the tumor cells remained more constant. The amount of immunoreactive MnSOD was measured by Western blotting. In all cases studied, the amount of immunoreactive MnSOD was lower in the transformed cells than in the normal cells.
对W138人胚肺成纤维细胞和经SV40转化的WI38细胞的锰超氧化物歧化酶（MnSOD）活性进行了测定。在各种生长条件下，转化细胞的MnSOD活性始终低于其正常细胞对应物。WI38细胞中MnSOD的活性随生长条件变化很大，而肿瘤细胞中的MnSOD活性则保持相对稳定。通过蛋白质印迹法测定免疫反应性MnSOD的量。在所有研究的案例中，转化细胞中免疫反应性MnSOD的量低于正常细胞。
