Garai Ellis, Sensarn Steven, Zavaleta Cristina L, Loewke Nathan O, Rogalla Stephan, Mandella Michael J, Felt Stephen A, Friedland Shai, Liu Jonathan T C, Gambhir Sanjiv S, Contag Christopher H
Department of Mechanical Engineering, Stanford University, Stanford, California, United States of America; Molecular Imaging Program at Stanford (MIPS), Stanford University, Stanford, California, United States of America.
Department of Radiology, Stanford University, Stanford, California, United States of America; Department of Pediatrics, Stanford University, Stanford, California, United States of America; Molecular Imaging Program at Stanford (MIPS), Stanford University, Stanford, California, United States of America.
PLoS One. 2015 Apr 29;10(4):e0123185. doi: 10.1371/journal.pone.0123185. eCollection 2015.
The detection of biomarker-targeting surface-enhanced Raman scattering (SERS) nanoparticles (NPs) in the human gastrointestinal tract has the potential to improve early cancer detection; however, a clinically relevant device with rapid Raman-imaging capability has not been described. Here we report the design and in vivo demonstration of a miniature, non-contact, opto-electro-mechanical Raman device as an accessory to clinical endoscopes that can provide multiplexed molecular data via a panel of SERS NPs. This device enables rapid circumferential scanning of topologically complex luminal surfaces of hollow organs (e.g., colon and esophagus) and produces quantitative images of the relative concentrations of SERS NPs that are present. Human and swine studies have demonstrated the speed and simplicity of this technique. This approach also offers unparalleled multiplexing capabilities by simultaneously detecting the unique spectral fingerprints of multiple SERS NPs. Therefore, this new screening strategy has the potential to improve diagnosis and to guide therapy by enabling sensitive quantitative molecular detection of small and otherwise hard-to-detect lesions in the context of white-light endoscopy.
在人体胃肠道中检测靶向生物标志物的表面增强拉曼散射（SERS）纳米颗粒（NPs）具有改善癌症早期检测的潜力；然而，尚未有具备快速拉曼成像能力的临床相关设备被报道。在此，我们报告了一种微型、非接触式光机电拉曼设备的设计及其体内演示，该设备作为临床内窥镜的附件，可通过一组SERS NPs提供多重分子数据。该设备能够对中空器官（如结肠和食道）拓扑结构复杂的管腔表面进行快速圆周扫描，并生成所存在的SERS NPs相对浓度的定量图像。人体和猪的研究已经证明了该技术的速度和简便性。这种方法还通过同时检测多个SERS NPs的独特光谱指纹提供了无与伦比的多重检测能力。因此，这种新的筛查策略有可能通过在白光内窥镜检查中对小的、难以检测的病变进行灵敏的定量分子检测来改善诊断并指导治疗。
