大疱性类天疱疮和寻常型天疱疮皮损中CD163 + CD206 + M2巨噬细胞的比较：大疱性类天疱疮可能的发病机制
Comparison of CD163+ CD206+ M2 macrophages in the lesional skin of bullous pemphigoid and pemphigus vulgaris: the possible pathogenesis of bullous pemphigoid.
作者信息
Furudate Sadanori, Fujimura Taku, Kambayashi Yumi, Kakizaki Aya, Aiba Setsuya
机构信息
Department of Dermatology, Tohoku University Graduate School of Medicine, Sendai, Japan.
出版信息
Dermatology. 2014;229(4):369-78. doi: 10.1159/000365946. Epub 2014 Nov 13.
BACKGROUND
M2 macrophages play a critical role in the induction of T helper 2 (Th2) polarization.
METHODS
To investigate the contribution of M2 macrophages to the pathogenesis of bullous pemphigoid (BP) and pemphigus vulgaris (PV), we employed immunohistochemical staining for CD163 and CD206, as well as pSTAT1, pSTAT6, interleukin (IL)-4, IL-13, CCL17, CCL18 and Foxp3 in the lesional skin of 10 cases of BP and PV.
RESULTS
The numbers of CD163+ CD206+ M2 macrophages were higher in BP than in PV. Moreover, pSTAT6+ cells, CCL17+ cells, CCL18+ cells and Foxp3+ regulatory T cells were prominent only in the lesional skin of BP. To further investigate the function of M2 macrophages, we examined the mRNA expression and production of Th2-related chemokines from M2 macrophages in vitro, which showed a significant increase in the mRNA expression and production of CCL18 when stimulated by IL-4 or IL-13.
CONCLUSION
Our study sheds light onto one of the possible immunological mechanisms of BP and PV.
背景
M2巨噬细胞在诱导辅助性T细胞2（Th2）极化中起关键作用。
方法
为研究M2巨噬细胞在大疱性类天疱疮（BP）和寻常型天疱疮（PV）发病机制中的作用，我们对10例BP和PV患者的皮损进行免疫组化染色，检测CD163、CD206以及pSTAT1、pSTAT6、白细胞介素（IL）-4、IL-13、CCL17、CCL18和Foxp3。
结果
BP患者皮损中CD163+CD206+M2巨噬细胞数量高于PV患者。此外，pSTAT6+细胞、CCL17+细胞、CCL18+细胞和Foxp3+调节性T细胞仅在BP患者皮损中显著。为进一步研究M2巨噬细胞的功能，我们检测了体外培养的M2巨噬细胞中Th2相关趋化因子的mRNA表达及分泌情况，结果显示，IL-4或IL-13刺激后，CCL18的mRNA表达及分泌显著增加。
结论
我们的研究揭示了BP和PV可能的免疫机制之一。
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