Prabakaran Sudhakaran, Hemberg Martin, Chauhan Ruchi, Winter Dominic, Tweedie-Cullen Ry Y, Dittrich Christian, Hong Elizabeth, Gunawardena Jeremy, Steen Hanno, Kreiman Gabriel, Steen Judith A
1] Proteomics Center, Boston Children's Hospital, Boston, Massachusetts 02115, USA [2] Department of Systems Biology, Harvard Medical School, Boston, Massachusetts 02115, USA.
Department of Ophthalmology, Boston Children's Hospital, Boston, Massachusetts 02115, USA.
Nat Commun. 2014 Nov 18;5:5429. doi: 10.1038/ncomms6429.
Only a small fraction of the mammalian genome codes for messenger RNAs destined to be translated into proteins, and it is generally assumed that a large portion of transcribed sequences--including introns and several classes of noncoding RNAs (ncRNAs)--do not give rise to peptide products. A systematic examination of translation and physiological regulation of ncRNAs has not been conducted. Here we use computational methods to identify the products of non-canonical translation in mouse neurons by analysing unannotated transcripts in combination with proteomic data. This study supports the existence of non-canonical translation products from both intragenic and extragenic genomic regions, including peptides derived from antisense transcripts and introns. Moreover, the studied novel translation products exhibit temporal regulation similar to that of proteins known to be involved in neuronal activity processes. These observations highlight a potentially large and complex set of biologically regulated translational events from transcripts formerly thought to lack coding potential.
只有一小部分哺乳动物基因组编码注定要被翻译成蛋白质的信使RNA，人们通常认为转录序列的很大一部分——包括内含子和几类非编码RNA（ncRNA）——不会产生肽产物。尚未对ncRNA的翻译和生理调节进行系统研究。在这里，我们使用计算方法，通过结合蛋白质组学数据分析未注释的转录本来鉴定小鼠神经元中非经典翻译的产物。这项研究支持了来自基因内和基因外基因组区域的非经典翻译产物的存在，包括源自反义转录本和内含子的肽。此外，所研究的新型翻译产物表现出与已知参与神经元活动过程的蛋白质相似的时间调节。这些观察结果突出了一组潜在的大量且复杂的受生物调节的翻译事件，这些事件来自以前被认为缺乏编码潜力的转录本。
