a Section of Environmental Health, Department of Public Health, University of Copenhagen , Copenhagen , Denmark.
Nanotoxicology. 2015;9(7):813-24. doi: 10.3109/17435390.2014.980449. Epub 2015 Sep 10.
The importance of composition, size, crystal structure, charge and coating of metal-based nanomaterials (NMs) were evaluated in human umbilical vein endothelial cells (HUVECs) and/or THP-1 monocytic cells. Biomarkers of oxidative stress and inflammation were assessed because they are important in the development of cardiovascular diseases. The NMs used were five TiO(2) NMs with different charge, size and crystal structure, coated and uncoated ZnO NMs and Ag which were tested in a wide concentration range. There were major differences between the types of NMs; exposure to ZnO and Ag resulted in cytotoxicity and increased gene expression levels of HMOX1 and IL8. The intracellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1(VCAM-1) expression were highest in TiO(2) NM-exposed cells. There was increased adhesion of THP-1 monocytic cells onto HUVECs with Ag exposure. None of the NMs increased the intracellular ROS production. There were no major effects of the coating of ZnO NMs. The TiO(2) NMs data on ICAM-1 and VCAM-1 expression suggested that the anatase form was more potent than the rutile form. In addition, the larger TiO(2) NM was more potent than the smaller for gene expression and ICAM-1 and VCAM-1 expression. The toxicological profile of cardiovascular disease-relevant biomarkers depended on composition, size and crystal structure of TiO(2) NMs, whereas the charge on TiO(2) NMs and the coating of ZnO NMs were not associated with differences in toxicological profile.
研究人员评估了金属基纳米材料（NMs）的组成、大小、晶体结构、电荷和涂层在人脐静脉内皮细胞（HUVECs）和/或 THP-1 单核细胞中的作用。因为氧化应激和炎症生物标志物在心血管疾病的发展中很重要，所以研究人员评估了它们。研究中使用了具有不同电荷、大小和晶体结构的五种 TiO2 NM、涂覆和未涂覆的 ZnO NM 和 Ag，并且测试了它们的广泛浓度范围。不同类型的 NM 之间存在很大差异；暴露于 ZnO 和 Ag 会导致细胞毒性，并增加 HMOX1 和 IL8 的基因表达水平。暴露于 TiO2 NM 的细胞中细胞间黏附分子-1（ICAM-1）和血管细胞黏附分子-1（VCAM-1）的表达最高。暴露于 Ag 会增加 THP-1 单核细胞与 HUVEC 的黏附。没有 NM 会增加细胞内 ROS 的产生。ZnO NM 的涂层没有主要影响。关于 ICAM-1 和 VCAM-1 表达的 TiO2 NM 数据表明，锐钛矿形式比金红石形式更有效。此外，较大的 TiO2 NM 比较小的 NM 在基因表达和 ICAM-1 和 VCAM-1 表达方面更有效。与心血管疾病相关的生物标志物的毒理学特征取决于 TiO2 NM 的组成、大小和晶体结构，而 TiO2 NM 的电荷和 ZnO NM 的涂层与毒理学特征的差异无关。
