基质血管成分辅助脂肪移植的体内生物成像分析：细胞与移植脂肪的相互作用和共生。
In vivo bioimaging analysis of stromal vascular fraction-assisted fat grafting: the interaction and mutualism of cells and grafted fat.
机构信息
1 Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. 2 Division of Development of Advanced Treatment, Center for Development of Advanced Medical Technology, Jichi Medical University, Tochigi, Japan. 3 Address correspondence to: Qing-Feng Li, M.D., Ph.D., Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Rd, Shanghai, 200011, China.
出版信息
Transplantation. 2014 Nov 27;98(10):1048-55. doi: 10.1097/TP.0000000000000397.
BACKGROUND
Unpredictable survival rate of transplanted fat is an obstacle in application of fat grafting. Although recent researches have suggested that adipose-derived stromal vascular fraction (SVF) could promote grafted fat survival, there has been seldom reports on tracing the dynamic change of grafted fat in vivo and on discussing interaction between transplanted SVFs and surrounding fat graft.
METHODS
Fat tissue and SVF separated from luciferase (Luc)-transgenic rats were applied for bioimaging analysis. The Luc-fat (0.2 mL) was subcutaneously injected into the back of nude mice with or without SVFs from 0.2 mL wild type rat fat, with bioimaging at 63 days. Immunohistochemical staining was performed to evaluate the structural integrity. Moreover, to evaluate the influence of surrounding fat tissue to transplanted SVFs, Luc-SVFs separated from 0.2 mL luciferase fat were transplanted to evaluate the influence of surrounding fat tissue to transplanted SVFs.
RESULTS
The bioimaging results showed that fat tissues transplanted with SVFs had higher survival ratio than those transplanted without SVFs (49.99(5.38)% vs. 32.78(3.32)%; P < 0.001). Stromal vascular fraction-assisted fat grafts had more integral structure and less necrosis cysts. The results showed that, with the existence of grafted fat, transplanted SVF survived for a significantly longer time and could contribute to fat graft survival and regeneration by differentiating into structural cells.
CONCLUSION
The results showed that SVF-assisted fat graft had significantly higher survival rate than that transplanted alone. Moreover, our research demonstrated that interaction between grafted fat and SVFs was important in SVF's long-term living and differentiation.
背景
移植脂肪存活率的不可预测性是脂肪移植应用的障碍。尽管最近的研究表明脂肪来源的基质血管部分（SVF）可以促进移植脂肪的存活，但关于体内追踪移植脂肪的动态变化以及讨论移植的 SVF 与周围脂肪移植物之间的相互作用的报道很少。
方法
从荧光素酶（Luc）转基因大鼠中分离脂肪组织和 SVF，进行生物成像分析。Luc 脂肪（0.2 mL）皮下注射到裸鼠背部，有无 SVF 来自 0.2 mL 野生型大鼠脂肪，63 天进行生物成像。进行免疫组织化学染色以评估结构完整性。此外，为了评估周围脂肪组织对移植 SVF 的影响，从 0.2 mL 荧光素酶脂肪中分离的 Luc-SVF 移植到评估周围脂肪组织对移植 SVF 的影响。
结果
生物成像结果表明，与未移植 SVF 的脂肪组织相比，移植 SVF 的脂肪组织存活率更高（49.99（5.38）％对 32.78（3.32）％；P <0.001）。基质血管部分辅助脂肪移植物具有更完整的结构和更少的坏死囊肿。结果表明，在存在移植物脂肪的情况下，移植的 SVF 存活时间明显更长，并且可以通过分化为结构细胞来促进脂肪移植物的存活和再生。
结论
结果表明，SVF 辅助脂肪移植物的存活率明显高于单独移植的脂肪移植物。此外，我们的研究表明，移植物脂肪和 SVF 之间的相互作用对于 SVF 的长期存活和分化很重要。
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