甲型肝炎病毒衣壳蛋白和RNA聚合酶编码cDNA的序列分析。
Sequence analysis of hepatitis A virus cDNA coding for capsid proteins and RNA polymerase.
作者信息
Baroudy B M, Ticehurst J R, Miele T A, Maizel J V, Purcell R H, Feinstone S M
出版信息
Proc Natl Acad Sci U S A. 1985 Apr;82(7):2143-7. doi: 10.1073/pnas.82.7.2143.
We report here the nucleotide sequence corresponding to two large regions of the hepatitis A virus (HAV) genome. These comprise a sequence of 3274 bases corresponding to the 5' end of the genome, which includes the putative capsid protein region of this picornavirus, and 1590 bases corresponding to the 3' end of the genome, terminating in a 15-base poly(A) tract. These sequences revealed that HAV had the characteristic genomic organization of picornaviruses: an open reading frame beginning approximately 750 bases from the 5' end of the RNA and a termination codon 60 bases from the 3' poly(A) tract. The predicted amino acid sequences of both regions have been compared to analogous regions previously determined for other picornaviruses. There was sufficient homology to conclude that the 5' region of HAV codes for capsid proteins and that the 3' region codes for an RNA polymerase. However, these regions of HAV were not found to be closely related to analogous regions of poliovirus, encephalomyocarditis virus, and foot and mouth disease virus.
我们在此报告与甲型肝炎病毒（HAV）基因组两个大区域相对应的核苷酸序列。这些序列包括一段3274个碱基的序列，对应于基因组的5'端，其中包含这种小核糖核酸病毒的假定衣壳蛋白区域，以及1590个碱基的序列，对应于基因组的3'端，以一个15个碱基的聚腺苷酸尾结束。这些序列显示，HAV具有小核糖核酸病毒的典型基因组结构：一个开放阅读框从RNA的5'端大约750个碱基处开始，一个终止密码子在距3'聚腺苷酸尾60个碱基处。已将这两个区域预测的氨基酸序列与先前为其他小核糖核酸病毒确定的类似区域进行了比较。有足够的同源性可以得出结论，HAV的5'区域编码衣壳蛋白，3'区域编码RNA聚合酶。然而，未发现HAV的这些区域与脊髓灰质炎病毒、脑心肌炎病毒和口蹄疫病毒的类似区域密切相关。
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