Asare George Awuku, Afriyie Dan, Ngala Robert A, Abutiate Harry, Doku Derek, Mahmood Seidu A, Rahman Habibur
University of Ghana, Accra, Ghana
University of Ghana, Accra, Ghana.
Integr Cancer Ther. 2015 Jan;14(1):65-74. doi: 10.1177/1534735414550198. Epub 2014 Nov 18.
Annona muricata L. has been reported to possess antitumor and antiproliferative properties. Not much work has been done on its effect on BPH-1 cell lines, and no in vivo studies targeting the prostate organ exist. The study determined the effect of A muricata on human BPH-1 cells and prostate organ.
The MTT assay was performed on BPH-1 cells using the aqueous leaf extract of A muricata. Cells (1 × 10(5) per well) were challenged with 0.5, 1.0, and 1.5 mg/mL extract for 24, 48, and 72 hours. Cell proliferation and morphology were examined microscopically. BPH-1 cells (1 × 10(4) per well) were seeded into 6-well plates and incubated for 48 hours with 0.5, 1.0, and 1.5 mg/mL A muricata extract. Reverse transcriptase polymerase chain reaction was performed using mRNA extracted from the cells. Possible target genes, Bax and Bcl-2, were examined. Twenty F344 male rats (≈200 g) were gavaged 30 mg/mL (10 rats) and 300 mg/mL (10 rats) and fed ad libitum alongside 10 control rats. Rats were sacrificed after 60 days. The prostate, seminal vesicles, and testes were harvested for histological examination.
Annona muricata demonstrated antiproliferative effects with an IC50 of 1.36 mg/mL. Best results were obtained after 48 hours, with near cell extinction at 72 hours. Bax gene was upregulated, while Bcl-2 was downregulated. Normal histological architecture was observed for all testes. Seminal vesicle was significantly reduced in test groups (P < .05) and demonstrated marked atrophy with increased cellularity and the acinii, empty of secretion. Prostate of test groups were reduced with epithelial lining showing pyknotic nucleus, condensation, and marginalization of the nuclear material, characteristic of apoptosis of the glandular epithelium. Furthermore, scanty prostatic secretion with flattening of acinar epithelial lining occurred.
Annona muricata has antiproliferative effects on BPH-1 cells and reduces prostate size, possibly through apoptosis.
据报道，刺果番荔枝具有抗肿瘤和抗增殖特性。关于其对BPH - 1细胞系的影响研究较少，且不存在针对前列腺器官的体内研究。本研究确定了刺果番荔枝对人BPH - 1细胞和前列腺器官的影响。
使用刺果番荔枝叶水提取物对BPH - 1细胞进行MTT试验。将细胞（每孔1×10⁵个）分别用0.5、1.0和1.5 mg/mL的提取物处理24、48和72小时。通过显微镜检查细胞增殖和形态。将BPH - 1细胞（每孔1×10⁴个）接种到6孔板中，并用0.5、1.0和1.5 mg/mL的刺果番荔枝提取物孵育48小时。使用从细胞中提取的mRNA进行逆转录聚合酶链反应。检测可能的靶基因Bax和Bcl - 2。将20只F344雄性大鼠（约200 g）分别灌胃30 mg/mL（10只大鼠）和300 mg/mL（10只大鼠），并与10只对照大鼠一起自由进食。60天后处死大鼠。采集前列腺、精囊和睾丸进行组织学检查。
刺果番荔枝表现出抗增殖作用，IC50为1.36 mg/mL。48小时后获得最佳结果，72小时时细胞接近灭绝。Bax基因上调，而Bcl - 2基因下调。所有睾丸均观察到正常的组织结构。试验组精囊明显减小（P <.05），表现出明显萎缩，细胞增多，腺泡无分泌物。试验组前列腺减小，上皮衬里显示细胞核固缩、核物质凝聚和边缘化，这是腺上皮细胞凋亡的特征。此外，腺泡上皮衬里变平，前列腺分泌物稀少。
刺果番荔枝对BPH - 1细胞具有抗增殖作用，并可能通过细胞凋亡减小前列腺大小。
