Lee Juneyoung, Lee Dong Gun
School of Life Sciences, BK 21 Plus KNU Creative BioResearch Group, College of Natural Sciences, Kyungpook National University, Daehak-ro 80, Buk-gu, Daegu, 702-701, Republic of Korea.
Curr Microbiol. 2015 Mar;70(3):383-9. doi: 10.1007/s00284-014-0734-1. Epub 2014 Nov 21.
Resveratrol (3,5,4'-trihydroxy-trans-stilbene) is a well-known natural polyphenolic compound that has garnered considerable interest because of its bioavailability and pharmacokinetics in humans. Although the antimicrobial activity of resveratrol has recently been focused, however, the antifungal activity and its mechanism are still largely unknown. Here, we report for the first time the potential of resveratrol as an apoptosis inducer in the human pathogenic fungus Candida albicans. The results showed that resveratrol exerted its effects from the early to the late stages of apoptosis and involved the activity of reactive oxygen species, particularly hydroxyl radicals ((∙)OH). DiOC6(3) and JC-1 staining indicated that loss of mitochondrial membrane potential (ΔΨ m) is a key event in resveratrol-induced apoptosis. Finally, we investigated metacaspase activation resulting from mitochondrial dysfunction. The result showed that resveratrol strongly activated metacaspase and promoted cytochrome c release. In summary, resveratrol induces fungal apoptosis through a caspase-dependent mitochondrial pathway and is a potential agent for treating human fungal diseases.
白藜芦醇（3,5,4'-三羟基反式芪）是一种著名的天然多酚化合物，因其在人体内的生物利用度和药代动力学而备受关注。尽管白藜芦醇的抗菌活性最近受到关注，但其抗真菌活性及其机制仍 largely unknown。在此，我们首次报道了白藜芦醇作为人类致病真菌白色念珠菌凋亡诱导剂的潜力。结果表明，白藜芦醇在凋亡的早期到晚期发挥作用，并涉及活性氧的活性，特别是羟基自由基（(∙)OH）。DiOC6(3)和JC-1染色表明线粒体膜电位（ΔΨ m）的丧失是白藜芦醇诱导凋亡的关键事件。最后，我们研究了线粒体功能障碍导致的 metacaspase 激活。结果表明，白藜芦醇强烈激活 metacaspase 并促进细胞色素 c 释放。总之，白藜芦醇通过 caspase 依赖的线粒体途径诱导真菌凋亡，是治疗人类真菌疾病的潜在药物。
