Banga S S, Boyd J B, Valerie K, Harris P V, Kurz E M, de Riel J K
Department of Genetics, University of California, Davis 95616.
Proc Natl Acad Sci U S A. 1989 May;86(9):3227-31. doi: 10.1073/pnas.86.9.3227.
The denV gene of bacteriophage T4 was fused to a Drosophila hsp70 (70-kDa heat shock protein) promoter and introduced into the germ line of Drosophila by P-element-mediated transformation. The protein product of that gene (endonuclease V) was detected in extracts of heat-shocked transformants with both enzymological and immunoblotting procedures. That protein restores both excision repair and UV resistance to mei-9 and mus201 mutants of this organism. These results reveal that the denV gene can compensate for excision-repair defects in two very different eukaryotic mutants, in that the mus201 mutants are typical of excision-deficient mutants in other organisms, whereas the mei-9 mutants exhibit a broad pleiotropism that includes a strong meiotic deficiency. This study permits an extension of the molecular analysis of DNA repair to the germ line of higher eukaryotes. It also provides a model system for future investigations of other well-characterized microbial repair genes on DNA damage in the germ line of this metazoan organism.
噬菌体T4的denV基因与果蝇hsp70（70 kDa热休克蛋白）启动子融合，并通过P因子介导的转化导入果蝇的种系。通过酶学和免疫印迹方法在热休克转化体的提取物中检测到该基因的蛋白质产物（核酸内切酶V）。该蛋白质可恢复该生物体的mei-9和mus201突变体的切除修复和紫外线抗性。这些结果表明，denV基因可以补偿两种非常不同的真核突变体中的切除修复缺陷，其中mus201突变体是其他生物体中典型的切除缺陷突变体，而mei-9突变体表现出广泛的多效性，包括强烈的减数分裂缺陷。这项研究允许将DNA修复的分子分析扩展到高等真核生物的种系。它还为未来研究其他特征明确的微生物修复基因对这种后生动物种系中DNA损伤的影响提供了一个模型系统。
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