Lashgari M S, Tada H, Amini S, Khalili K
Department of Biochemistry and Molecular Biology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania 19107.
Virology. 1989 May;170(1):292-5. doi: 10.1016/0042-6822(89)90381-4.
To better understand the basis of cell type specificity of JCV replication, we have analyzed the expression of the viral late promoter in glial cells. Using transient transfection procedures, we show that the late gene expression, like that of the early gene, is restricted to glial cells. However, cotransfection with a plasmid producing the JCV early protein, T-antigen, stimulates expression from the JCV late promoter in both glial and non-glial cells. The SV40-encoded T-antigen acts similarly on transcription of JCV late promoter in both cell types. This transacting effect occurs at the level of RNA synthesis, as measured by the rate of transcription, stability of the message, and translation. These results indicate that basal JCV late promoter activity is restricted to glial cells, whereas in the presence of viral early protein this promoter functions in both glial and non-glial cells.
为了更好地理解JC病毒复制的细胞类型特异性基础，我们分析了病毒晚期启动子在神经胶质细胞中的表达。通过瞬时转染程序，我们发现晚期基因的表达，如同早期基因一样，局限于神经胶质细胞。然而，与产生JC病毒早期蛋白T抗原的质粒共转染，可刺激神经胶质细胞和非神经胶质细胞中JC病毒晚期启动子的表达。SV40编码的T抗原在这两种细胞类型中对JC病毒晚期启动子的转录作用相似。这种反式作用发生在RNA合成水平，通过转录速率、信使RNA的稳定性和翻译来衡量。这些结果表明，JC病毒晚期启动子的基础活性局限于神经胶质细胞，而在病毒早期蛋白存在的情况下，该启动子在神经胶质细胞和非神经胶质细胞中均发挥作用。
