通过结构照明显微镜数据的时空图像相关性对衍射极限之外的分子流进行量化。
Molecular flow quantified beyond the diffraction limit by spatiotemporal image correlation of structured illumination microscopy data.
作者信息
Ashdown George W, Cope Andrew, Wiseman Paul W, Owen Dylan M
机构信息
Department of Physics and Randall Division of Cell and Molecular Biophysics, King's College London, London, United Kingdom.
Academic Department of Rheumatology, Centre for Molecular and Cellular Biology of Inflammation, Division of Immunology, Infection and Inflammatory Disease, King's College London, London, United Kingdom.
出版信息
Biophys J. 2014 Nov 4;107(9):L21-3. doi: 10.1016/j.bpj.2014.09.018.
Abstract
We combine total internal reflection fluorescence structured illumination microscopy with spatiotemporal image correlation spectroscopy to quantify the flow velocities and directionality of filamentous-actin at the T cell immunological synapse. These techniques demonstrate it is possible to image retrograde flow of filamentous-actin at superresolution and provide flow quantification in the form of velocity histograms and flow vector maps. The flow was found to be retrograde and radially directed throughout the periphery of T-cells during synapse formation.
摘要
我们将全内反射荧光结构照明显微镜与时空图像相关光谱相结合，以量化丝状肌动蛋白在T细胞免疫突触处的流速和方向性。这些技术表明，可以以超分辨率对丝状肌动蛋白的逆行流动进行成像，并以速度直方图和流动矢量图的形式提供流动定量分析。研究发现，在突触形成过程中，T细胞周边的流动是逆行且呈放射状的。
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