Institute of Applied Physics - Biophysics, TU Wien, 1040 Vienna, Austria.
Humboldt-Universität zu Berlin, Institut für Biophysik, Experimentelle Biophysik Mechanobiologie, Sitz Invalidenstrasse 42, 10115 Berlin, Germany.
Biochem Soc Trans. 2022 Apr 29;50(2):853-866. doi: 10.1042/BST20210479.
Advanced imaging is key for visualizing the spatiotemporal regulation of immune signaling which is a complex process involving multiple players tightly regulated in space and time. Imaging techniques vary in their spatial resolution, spanning from nanometers to micrometers, and in their temporal resolution, ranging from microseconds to hours. In this review, we summarize state-of-the-art imaging methodologies and provide recent examples on how they helped to unravel the mysteries of immune signaling. Finally, we discuss the limitations of current technologies and share our insights on how to overcome these limitations to visualize immune signaling with unprecedented fidelity.
高级成像对于可视化免疫信号的时空调节至关重要，这是一个涉及多个参与者的复杂过程，在空间和时间上受到严格调控。成像技术在空间分辨率上有所不同，范围从纳米到微米，在时间分辨率上，从微秒到小时不等。在这篇综述中，我们总结了最先进的成像方法，并提供了最近的例子，说明它们如何帮助揭示免疫信号的奥秘。最后，我们讨论了当前技术的局限性，并分享了我们的见解，即如何克服这些限制，以前所未有的精度可视化免疫信号。
