Chen Hannah, Thorne Stephen H
Departments of Surgery and Immunology, University of Pittsburgh Cancer Institute University of Pittsburgh, Pittsburgh, Pennsylvania, Phone 412 623 4896, Fax 412 623 7709.
Curr Protoc Cytom. 2012;59(1224):12.24.1-12.24.11. doi: 10.1002/0471142956.cy1224s59.
Traditional approaches for translating observations of molecular events into the context of a living organism have suffered from the requirements for either sacrificing animals at multiple time points prior to labor-intensive analyses of multiple tissues, or have relied on subjective observations or measurements of the animals over time. Recently an explosion of dedicated animal imaging modalities and the release of modified clinical imaging devices dedicated for animal imaging have allowed for the design of quantitative real time experiments incorporating fewer animals and providing whole animal analyses. Of these modalities, optical imaging (bioluminescence and fluorescence) has emerged as a powerful research tool, allowing investigators with limited whole animal imaging expertise to rapidly and inexpensively translate models produced in cellular assays into the context of a living animal. Here we will outline the steps necessary for translation of models established in culture systems into rodents.
将分子事件的观察结果转化到活体生物环境中的传统方法，要么需要在对多个组织进行耗时费力的分析之前，在多个时间点处死动物，要么依赖于对动物随时间进行的主观观察或测量。最近，专用动物成像模式的激增以及专为动物成像设计的改良临床成像设备的推出，使得能够设计出包含较少动物并提供全动物分析的定量实时实验。在这些模式中，光学成像（生物发光和荧光）已成为一种强大的研究工具，使全动物成像专业知识有限的研究人员能够快速且低成本地将细胞实验中产生的模型转化到活体动物环境中。在此，我们将概述把在培养系统中建立的模型转化到啮齿动物中的必要步骤。
