So Hongyun, Lee Kunwoo, Murthy Niren, Pisano Albert P
Department of Mechanical Engineering, Berkeley Sensor & Actuator Center and ‡Department of Bioengineering, University of California , Berkeley, California 94720, United States.
ACS Appl Mater Interfaces. 2014 Dec 10;6(23):20693-9. doi: 10.1021/am506153y. Epub 2014 Nov 24.
This paper describes a handheld device that uses an all-in-one membrane for continuous mechanical cell lysis and rapid DNA isolation without the assistance of power sources, lysis reagents, and routine centrifugation. This nanowire-decorated multifunctional membrane was fabricated to isolate DNA by selective adsorption to silica surface immediately after disruption of nucleus membranes by ultrasharp tips of nanowires for a rapid cell lysis, and it can be directly assembled with commercial syringe filter holders. The membrane was fabricated by photoelectrochemical etching to create microchannel arrays followed by hydrothermal synthesis of nanowires and deposition of silica. The proposed membrane successfully purifies high-quality DNA within 5 min, whereas a commercial purification kit needs more than an hour.
本文描述了一种手持设备，该设备使用一体化膜进行连续的机械细胞裂解和快速DNA分离，无需电源、裂解试剂和常规离心操作。这种装饰有纳米线的多功能膜通过纳米线的超尖端部破坏细胞核膜后立即选择性吸附到二氧化硅表面来分离DNA，从而实现快速细胞裂解，并且它可以直接与商用注射器滤器支架组装。该膜通过光电化学蚀刻制造微通道阵列，然后进行纳米线的水热合成和二氧化硅的沉积。所提出的膜在5分钟内成功纯化出高质量的DNA，而商用纯化试剂盒则需要一个多小时。
