Department of Chemistry & Biochemistry, University of Maryland, College Park, MD 20742, USA.
Nanomedicine (Lond). 2014;9(8):1171-80. doi: 10.2217/nnm.13.96. Epub 2013 Jul 31.
To construct a new robust nanowire-based pellicle for eukaryotic cells, to investigate the interactions between nanowires (NWs) and cell surfaces and the internalization of nanowires, and to demonstrate for isolation of the plasma membrane with improved enrichment of transmembrane proteins.
MATERIALS & METHODS: Silica NWs were coated with alumina to give positive charges on their surface. Multiple myeloma cells were coated with the positively charged NWs by dropping the cells into a buffered suspension of NWs. After the NW-coated cells were lysed, plasma membrane fragments were enriched by differential centrifugation for proteomic studies.
Here we demonstrate complete cell coating with positively charged, alumina-coated silica NWs via nonspecific electrostatic interactions, and characterize a robust pellicle and little/no uptake of NWs.
Robust pellicles provide a new platform for therapeutic, diagnostic and biochemical interactions of nanostructures with eukaryotic cells.
构建一种新的基于纳米线的用于真核细胞的坚韧膜，研究纳米线（NWs）与细胞表面的相互作用和纳米线的内化，并展示用于分离质膜，同时改善跨膜蛋白的富集。
在硅纳米线的表面覆盖氧化铝以赋予其表面正电荷。通过将细胞滴入 NWs 的缓冲悬浮液中，多发性骨髓瘤细胞被带有正电荷的 NWs 包裹。在 NW 包裹的细胞裂解后，通过差速离心来富集质膜片段，以进行蛋白质组学研究。
我们通过非特异性静电相互作用证明了带正电荷的氧化铝覆盖的硅纳米线对细胞的完全包裹，并对坚韧的膜进行了表征，同时发现纳米线几乎没有被摄取。
坚韧的膜为纳米结构与真核细胞的治疗、诊断和生化相互作用提供了一个新的平台。
