Fischer Melina, Freuling Conrad M, Müller Thomas, Schatz Juliane, Rasmussen Thomas Bruun, Chriel Mariann, Balkema-Buschmann Anne, Beer Martin, Hoffmann Bernd
Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Südufer 10, D-17493, Greifswald-Insel Riems, Germany.
Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Südufer 10, D-17493, Greifswald-Insel Riems, Germany.
Virol J. 2014 Nov 25;11:196. doi: 10.1186/s12985-014-0196-x.
In the frame of active lyssavirus surveillance in bats, oropharyngeal swabs from German (N = 2297) and Danish (N = 134) insectivorous bats were investigated using a newly developed generic pan-lyssavirus real-time reverse transcriptase PCR (RT-qPCR).
In total, 15 RT-qPCR positive swabs were detected. Remarkably, sequencing of positive samples did not confirm the presence of bat associated lyssaviruses but revealed nine distinct novel rhabdovirus-related sequences.
Several novel rhabdovirus-related sequences were detected both in German and Danish insectivorous bats. The results also prove that the novel generic pan-lyssavirus RT-qPCR offers a very broad detection range that allows the collection of further valuable data concerning the broad and complex diversity within the family Rhabdoviridae.
在对蝙蝠进行狂犬病病毒主动监测的框架下,使用新开发的通用型狂犬病病毒实时逆转录聚合酶链反应(RT-qPCR)对来自德国(N = 2297)和丹麦(N = 134)的食虫蝙蝠的口咽拭子进行了调查。
总共检测到15份RT-qPCR阳性拭子。值得注意的是,对阳性样本进行测序并未确认存在与蝙蝠相关的狂犬病病毒,而是揭示了9个不同的新型弹状病毒相关序列。
在德国和丹麦的食虫蝙蝠中均检测到了几种新型弹状病毒相关序列。结果还证明,新型通用型狂犬病病毒RT-qPCR具有非常广泛的检测范围,能够收集有关弹状病毒科广泛而复杂的多样性的更多有价值的数据。