Am J Trop Med Hyg. 2014 Aug;91(2):258-266. doi: 10.4269/ajtmh.13-0664. Epub 2014 May 27.
We screened 217 bats of at least 20 species from 17 locations in Kenya during July and August of 2006 for the presence of adenovirus, rhabdovirus, and paramyxovirus nucleic acids using generic reverse transcription polymerase chain reaction (RT-PCR) and PCR assays. Of 217 bat fecal swabs examined, 4 bats were adenovirus DNA-positive, 11 bats were paramyxovirus RNA-positive, and 2 bats were rhabdovirus RNA-positive. Three bats were coinfected by two different viruses. By sequence comparison and phylogenetic analysis, the Kenya bat paramyxoviruses and rhabdoviruses from this study may represent novel viral lineages within their respective families; the Kenya bat adenoviruses could not be confirmed as novel, because the same region sequences from other known bat adenovirus genomes for comparison were lacking. Our study adds to previous evidence that bats carry diverse, potentially zoonotic viruses and may be coinfected with more than one virus.
我们在 2006 年 7 月至 8 月期间，从肯尼亚的 17 个地点筛选了至少 20 种 217 只蝙蝠，使用通用逆转录聚合酶链反应（RT-PCR）和 PCR 检测方法，检测腺病毒、弹状病毒和副粘病毒核酸的存在情况。在检查的 217 份蝙蝠粪便拭子中，有 4 份蝙蝠的腺病毒 DNA 呈阳性，11 份蝙蝠的副粘病毒 RNA 呈阳性，2 份蝙蝠的弹状病毒 RNA 呈阳性。有 3 只蝙蝠同时感染了两种不同的病毒。通过序列比较和系统发育分析，本研究中的肯尼亚蝙蝠副粘病毒和弹状病毒可能代表各自科内的新型病毒谱系；肯尼亚蝙蝠腺病毒不能被确认为新型病毒，因为缺乏与其他已知蝙蝠腺病毒基因组进行比较的同一区域序列。我们的研究增加了之前的证据，表明蝙蝠携带多种潜在的人畜共患病病毒，并且可能同时感染多种病毒。
