Harris S L, Aegerter J N, Brookes S M, McElhinney L M, Jones G, Smith G C, Fooks A R
School of Biological Sciences, University of Bristol, Woodland Road, Bristol, BS8 1UG, UK.
J Wildl Dis. 2009 Oct;45(4):1030-41. doi: 10.7589/0090-3558-45.4.1030.
In 2003-06, targeted (active) surveillance for European bat lyssaviruses (EBLVs) was undertaken throughout England, focusing on two species most likely to host these viruses, Myotis daubentonii and Eptesicus serotinus. Blood was sampled for the detection of EBLV-specific neutralizing antibodies and oropharyngeal swabs were taken for the detection of viral RNA or infectious virus in saliva. Between 2003 and 2006, 273 E. serotinus and 363 M. daubentonii blood samples were tested by the EBLV-1 or EBLV-2 specific modified fluorescent antibody neutralization test. The EBLV-2 antibody prevalence estimate was 1.0-4.1% (95% confidence interval [CI]; mean=2.2%) for M. daubentonii. European bat lyssavirus type 1-specific antibodies were detected only in a single E. serotinus. Other nontarget species (n=5) were sampled in small numbers (n=24), with no EBLV-specific antibody detected. No viral RNA or live virus was detected in any of the oropharyngeal swabs analyzed. Host RNA was detected from 83% of the oropharyngeal swabs analyzed (total swabs 2003-06: n=766). These data show that EBLV-2 is present in M. daubentonii in England. In contrast, there is insufficient evidence to suggest that EBLV-1 is present in E. serotinus in England, although further research is warranted.
2003年至2006年期间，在英格兰全境对欧洲蝙蝠狂犬病病毒（EBLVs）开展了目标性（主动）监测，重点关注最有可能携带这些病毒的两种蝙蝠，即道氏鼠耳蝠和血清型棕蝠。采集血液样本以检测EBLV特异性中和抗体，并采集口咽拭子以检测唾液中的病毒RNA或传染性病毒。2003年至2006年期间，采用EBLV-1或EBLV-2特异性改良荧光抗体中和试验对273份血清型棕蝠和363份道氏鼠耳蝠的血液样本进行了检测。道氏鼠耳蝠的EBLV-2抗体流行率估计为1.0%-4.1%（95%置信区间[CI]；平均值=2.2%）。仅在一只血清型棕蝠中检测到1型欧洲蝙蝠狂犬病病毒特异性抗体。其他非目标物种（n=5）采样数量较少（n=24），未检测到EBLV特异性抗体。在分析的任何口咽拭子中均未检测到病毒RNA或活病毒。在分析的83%的口咽拭子中检测到宿主RNA（2003年至2006年拭子总数：n=766）。这些数据表明，EBLV-2存在于英格兰的道氏鼠耳蝠中。相比之下，虽然有必要进行进一步研究，但没有足够证据表明EBLV-1存在于英格兰的血清型棕蝠中。
