瓦尼针层孔菌的菌丝体发酵特性及抗增殖活性

Mycelial fermentation characteristics and antiproliferative activity of Phellinus vaninii Ljup.

作者信息

Hu Wei, Liu Shuai, Zhang Yuexin, Xun Deng, Xu Chunping

机构信息

Branch of Forest Protection, School of Forestry, Northeast Forestry University, Mudanjiang 157011, China ; Heilongjiang Forest By-product and Specialty Institute, Mudanjiang 157011, China.

College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, Henan 450002, China.

出版信息

Pharmacogn Mag. 2014 Oct;10(40):430-4. doi: 10.4103/0973-1296.141812.

DOI:10.4103/0973-1296.141812

PMID:25422542

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4239719/

Abstract

BACKGROUND

The mycelial fermentation of higher fungi were investigated to posses various bioactivities.

MATERIALS AND METHODS

The mycelial growth and pellet morphology in a 5-L bioreactor were investigated. The mycelial broth containing biomass and extracellular products harvested from the fermentor was tested for antiproliferative activity of colon cancer LoVo cells using 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide assay.

RESULTS

The maximum mycelial concentration in a 5-L bioreactor was 12.5 g/L after 8 days cultivation. Further investigation in the mycelial pellets during the fermentation period revealed that the mean diameter of the pellet morphology was positively correlated with mycelial biomass (R 2 = 0.82, P < 0.05) and broth viscosity (R 2 = 0.90, P < 0.01), significantly. The ethyl acetate extract showed the most significant effects, increasing the inhibition rate up to 87.5% after 48 h at concentration of 1000 μg/mL.

CONCLUSION

The results demonstrated the feasibility of P. vaninii Ljup mycelial fermentation for large-scale production of bioactive and medicinal compounds.

摘要

背景

对高等真菌的菌丝体发酵进行了研究，以了解其具有的各种生物活性。

材料与方法

研究了5升生物反应器中菌丝体的生长和颗粒形态。使用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐法，对从发酵罐收获的含有生物质和细胞外产物的菌丝体肉汤进行结肠癌LoVo细胞的抗增殖活性测试。

结果

在5升生物反应器中培养8天后，最大菌丝体浓度为12.5克/升。对发酵期间菌丝体颗粒的进一步研究表明，颗粒形态的平均直径与菌丝体生物量（R² = 0.82，P < 0.05）和肉汤粘度（R² = 0.90，P < 0.01）呈显著正相关。乙酸乙酯提取物显示出最显著的效果，在浓度为1000微克/毫升时，48小时后抑制率提高到87.5%。