Im Kyung Hoan, Baek Seung A, Choi Jaehyuk, Lee Tae Soo
Division of Life Sciences, Incheon National University, Incheon, Korea.
Mycobiology. 2019 Oct 15;47(4):494-505. doi: 10.1080/12298093.2019.1673595. eCollection 2019.
In this study, the antioxidant, anti-xanthine oxidase, anti-melanogenic and anti-wrinkle effects of methanol (ME) and hot water (HE) extracts from the fruiting bodies of were investigated. The 1,1-diphenyl-2-picryl-hydrazyl free radical scavenging activity of 2.0 mg/mL HE (95.38%) was comparable to that of butylated hydroxytoluene (96.97%), the reference standard. The hydroxyl radical scavenging activities of ME (98.19%) and HE (97.55%) were higher than that of butylated hydroxytoluene (92.66%) at 2.0 mg/mL. Neither ME nor HE was cytotoxic to murine melanoma B16-F10 cells at 25-750 µg/mL. Although the xanthine oxidase (XO) inhibitory effects of ME and HE were significantly lower than that of allopurinol, the values were higher than 84 percent. The tyrosinase inhibitory activities of ME and HE were comparable to kojic acid at 2.0 mg/mL. The cellular tyrosinase and melanin synthetic activities of ME and HE on B16-F10 melanoma cells at 500 µg/mL were higher than arbutin, indicating that the inhibitory effects of arbutin on the tyrosinase and melanin synthesis were higher than those of ME and HE. The collagenase inhibitory activity of HE was comparable to EGCG at 2.0 mg/mL, however, the elastase inhibitory activity of ME and HE was lower than EGCG at the concentration tested. The study results demonstrated that the fruiting bodies of . possessed good antioxidant, anti-xanthine oxidase, cell-free anti-tyrosinase, cellular anti-tyrosinase, anti-collagenase, and moderate anti-elastase activities, which might be used for the development of novel anti-gout, skin-whitening, and skin anti-wrinkle agents.
在本研究中，对[具体物种]子实体的甲醇提取物（ME）和热水提取物（HE）的抗氧化、抗黄嘌呤氧化酶、抗黑色素生成和抗皱作用进行了研究。2.0 mg/mL HE的1,1-二苯基-2-苦基肼自由基清除活性（95.38%）与参考标准丁基化羟基甲苯（96.97%）相当。在2.0 mg/mL时，ME（98.19%）和HE（97.55%）的羟基自由基清除活性高于丁基化羟基甲苯（92.66%）。在25 - 750 µg/mL时，ME和HE对小鼠黑色素瘤B16 - F10细胞均无细胞毒性。虽然ME和HE对黄嘌呤氧化酶（XO）的抑制作用明显低于别嘌呤醇，但其值高于84%。在2.0 mg/mL时，ME和HE的酪氨酸酶抑制活性与曲酸相当。ME和HE在500 µg/mL时对B16 - F10黑色素瘤细胞的细胞酪氨酸酶和黑色素合成活性高于熊果苷，表明熊果苷对酪氨酸酶和黑色素合成的抑制作用高于ME和HE。在2.0 mg/mL时，HE的胶原酶抑制活性与表没食子儿没食子酸酯（EGCG）相当，然而，在测试浓度下，ME和HE的弹性蛋白酶抑制活性低于EGCG。研究结果表明，[具体物种]的子实体具有良好的抗氧化、抗黄嘌呤氧化酶、无细胞抗酪氨酸酶、细胞抗酪氨酸酶、抗胶原酶和适度的抗弹性蛋白酶活性，可用于开发新型抗痛风、皮肤美白和皮肤抗皱剂。
