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gH625肽功能化树枝状大分子与脂质体相互作用机制的阐明。

Elucidation of the interaction mechanism with liposomes of gH625-peptide functionalized dendrimers.

作者信息

Falanga Annarita, Tarallo Rossella, Carberry Thomas, Galdiero Massimiliano, Weck Marcus, Galdiero Stefania

机构信息

Department of Pharmacy & CIRPEB & DFM Scarl, University of Naples "Federico II", Naples, Italy.

Molecular Design Institute and Department of Chemistry, New York University, New York, New York, United States of America.

出版信息

PLoS One. 2014 Nov 25;9(11):e112128. doi: 10.1371/journal.pone.0112128. eCollection 2014.

DOI:10.1371/journal.pone.0112128
PMID:25423477
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4244103/
Abstract

We have demonstrated that amide-based dendrimers functionalized with the membrane-interacting peptide gH625 derived from the herpes simplex virus type 1 (HSV-1) envelope glycoprotein H enter cells mainly through a non-active translocation mechanism. Herein, we investigate the interaction between the peptide-functionalized dendrimer and liposomes composed of PC/Chol using fluorescence spectroscopy, isothermal titration calorimetry, and surface plasmon resonance to get insights into the mechanism of internalization. The affinity for the membrane bilayer is very high and the interaction between the peptide-dendrimer and liposomes took place without evidence of pore formation. These results suggest that the presented peptidodendrimeric scaffold may be a promising material for efficient drug delivery.

摘要

我们已经证明，用源自单纯疱疹病毒1型（HSV-1）包膜糖蛋白H的膜相互作用肽gH625功能化的酰胺基树枝状大分子主要通过非主动转运机制进入细胞。在此，我们使用荧光光谱、等温滴定量热法和表面等离子体共振研究了肽功能化树枝状大分子与由PC/Chol组成的脂质体之间的相互作用，以深入了解内化机制。对膜双层的亲和力非常高，肽-树枝状大分子与脂质体之间的相互作用发生时没有形成孔的迹象。这些结果表明，所呈现的肽树枝状大分子支架可能是一种用于高效药物递送的有前途的材料。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aea4/4244103/7d1e1480ad1e/pone.0112128.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aea4/4244103/6b915f1501c6/pone.0112128.g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aea4/4244103/fb7f6593502a/pone.0112128.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aea4/4244103/a69cd30ce3e8/pone.0112128.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aea4/4244103/6d232294a795/pone.0112128.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aea4/4244103/546691375098/pone.0112128.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aea4/4244103/7d1e1480ad1e/pone.0112128.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aea4/4244103/6b915f1501c6/pone.0112128.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aea4/4244103/f72611e6acf7/pone.0112128.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aea4/4244103/6aeecb012372/pone.0112128.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aea4/4244103/4d1b212ecabb/pone.0112128.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aea4/4244103/fb7f6593502a/pone.0112128.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aea4/4244103/a69cd30ce3e8/pone.0112128.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aea4/4244103/6d232294a795/pone.0112128.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aea4/4244103/546691375098/pone.0112128.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aea4/4244103/7d1e1480ad1e/pone.0112128.g009.jpg

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gH625 is a viral derived peptide for effective delivery of intrinsically disordered proteins.gH625 是一种病毒衍生肽，可有效递呈天然无序蛋白。
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